乙型肝炎表面抗原联合rmIL-12诱导的小鼠细胞免疫应答  

Induction of Cellular Immune Response by HBsAg Combined with rmIL-12 in Mice

作　　者：傅泳航[1] 杨向阳[2] 李茹冰[1] 夏书奇 王翠玲 王增松 谢飞群 段祥 曾振飞 张宜俊 

机构地区：[1]解放军第458医院检验科,广州510602 [2]广州军区机关门诊部保健科,广州510080 [3]广州市恺泰生物技术公司,广州510620

出　　处：《中国生物制品学杂志》2009年第12期1199-1202,共4页Chinese Journal of Biologicals

基　　金：广东省科技计划项目基金(2007B060401053);广州经济技术开发区科技发展资金(2008Q-P064)

摘　　要：目的探讨乙型肝炎表面抗原(HBsAg)联合重组鼠白介素-12(rmIL-12)对免疫小鼠诱导细胞免疫应答的影响。方法以不同给药方式、不同剂量的HBsAg联合rmIL-12免疫C57BL/6J小鼠,ELISA法检测小鼠血清及脾淋巴细胞中IFNγ及IL-4的水平。结果HBsAg+rmIL-12高剂量组免疫的C57BL/6J小鼠,其脾淋巴细胞IFNγ水平明显高于BALB/c小鼠;3种不同给药方式均可诱导C57BL/6J小鼠血清和脾细胞IFNγ水平明显升高,三者之间差异无统计学意义;HBsAg+rmIL-12高、中、低剂量组免疫C57BL/6J小鼠血清可诱导IFNγ水平明显升高,且呈剂量依赖性,脾细胞IFNγ水平在中、低剂量组均未产生效应,高剂量组明显升高,而对IL-4无明显影响。结论rmIL-12可显著增强HBsAg诱导的细胞免疫应答,并使免疫应答转向Th1型,对于发展治疗性乙肝疫苗具有潜在的应用价值。Objective To investigate the effect of HBsAg combined with recombinant mouse IL-12（rmIL-12）on induction of cellular immune response in mice. Methods C57BL / 6J mice were immunized by HBsAg combined with rmIL-12, at various dosages by various routes, and the IFNγ and IL-4 levels in their sera and splenic lymophocytes were determined by ELISA. Results The IFNγ levels in splenic lymphocytes of C57BL / 6J mice immunized with HBsAg ＋ rmIL-12 at high dosage were significantly higher than those in splenic lymphocytes of BALB / c mice. The IFNγ levels in sera and splenic lymphocytes of C57BL / 6J mice immunized with HBsAg ＋ rmIL-12 by subcutaneous injection, HBsAg ＋ rmIL-12 by intramuscular injection and HBsAg by intramuscular injection ＋ rmIL-12 by subcutaneous injection increased significantly while showed no significant difference. All the IFNγ levels in sera of C57BL / 6J mice immunized with HBsAg ＋ rmIL-12 at high, moderate and low dosages increased significantly in a dose-dependent mode The IFNγ levels in splenic lymphocytes showed no significant change in the mice immunized with HBsAg ＋ rmIL-12 at moderate and low dosages, while increased significantly in those at high dosage. However, no significant effect on IL-4 level was observed. Conclusion Recombinant mouse IL-12 enhanced the cellular immune response level induced by HBsAg significantly and induced Th1 type immune response, which provided a basis for development of therapeutic hepatitis B vaccine.

关 键 词：乙型肝炎表面抗原 重组鼠白介素-12 细胞免疫 细胞因子 

分 类 号：R392.11[医药卫生—免疫学]