压力超负荷致心力衰竭大鼠解耦联蛋白2表达及心肌能量变化  

The relationship between uncoupfing protein 2 expression and myocardial high energy phosphates content in abdominal aorta constriction induced heart failure rats

作　　者：李楠[1] 王江[1] 高峰[1] 李爱民[1] 宋熔[1] 田颖[1] 祝善俊[1] 

机构地区：[1]第三军医大学新桥医院心内科,重庆400037

出　　处：《中华心血管病杂志》2009年第12期1108-1112,共5页Chinese Journal of Cardiology

摘　　要：目的探讨压力超负荷致心力衰竭大鼠心肌线粒体解耦联蛋白2（uncoupling protein2，UCP2）表达的改变及其意义。方法雄性SD大鼠45只随机分为腹主动脉缩窄组（H20w组）、假手术组（SH20w组）和正常对照组（N组）3组，每组15只，H20w组行腹主动脉缩窄术。术后20周，行心脏超声检测心功能、观察大鼠血液动力学参数，高压液相色谱法测量心肌组织腺苷酸含量，密度梯度法提取大鼠心肌线粒体，逆转录聚合酶链反应及Western blot法分析心肌线粒体UCP2表达。结果大鼠行腹主动脉缩窄术后20周出现心功能减退，血液动力学异常等心力衰竭表现，提示建模成功。同时间比较，H20w组心肌组织内三磷酸腺苷（ATP）、二磷酸腺苷、单磷酸腺苷及磷酸肌酸含量均低于SH20w组和N组。逆转录聚合酶链反应及Westernblot表明H20w组心肌线粒体UCP2表达水平分别较SH20w组高69％、61％，较N组高76％、65％（P〈0．01），相关性分析显示UCP2表达量与心肌组织内ATP含量变化呈负相关（r=-0．929，P〈0．01）。结论心力衰竭时，心肌线粒体UCP2表达增高，导致心肌ATP含量减少，可能参与了衰竭心肌能量代谢障碍。Objective To explore the changes of expression of uncoupling protein 2 （UCP2） in pressure overload induced failure myocardium in rats. Methods Male SD rats were randomized into 3 groups （n = 15 each） ： abdominal aorta constriction （AC） 20 weeks group （H20w group）, sham operation group （ SH20w group） and normal control group （ N group）. Twenty weeks later, myocardial function was evaluated by echocardiography and hemodynamic measurements. Mitochondria in ventricular tissue were isolated by centrifugation. Adenine nucleotide pools （ATP, ADP, AMP, PCr） in myocardium were measured by high performance liquid chromatography. The expression of UCP2 in mitochondria was detected by PT-PCR and Western blot analysis. Results Myocardial function was significantly decreased 20 weeks post-AC compared to SH20w group and N group. Myocardial ATP, ADP, AMP and PCr contents were also significantly decreased in H20w group than the other 2 control groups. The expression of UCP2 in myocardial mitochondria was significantly increased in H20w group and negatively correlated with ATP contents （r = -0.929 ,P 〈 0.01 ）. Conclusions The expression of UCP2 was upregulated in pressure overload induced failure heart and might be responsible for decreased myocardial adenine nucleotide and energy metabolism disturbance in this model.

关 键 词：心力衰竭 充血性 线粒体 能量代谢 解耦联蛋白2 

分 类 号：R541.6[医药卫生—心血管疾病]