葛根素预处理减轻大鼠心肌缺血再灌注损伤及PI3K/AKT信号通路的作用  

Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat

作　　者：马亚飞[1] 刘新伟[1] 文婷婷[1] 乔欣[1] 

机构地区：[1]重庆医科大学附属第一医院麻醉科,重庆400016

出　　处：《重庆医科大学学报》2009年第12期1673-1676,共4页Journal of Chongqing Medical University

摘　　要：目的:观察葛根素(Puerarin,Pue)预处理对缺血再灌注大鼠心肌的保护作用及其可能机制。方法:40只SD大鼠随机分为5组(n=8):假手术组(SH组);缺血再灌注组(I/R组);葛根素预处理组(PU组);葛根素预处理+LY294002组(PU+LY组);缺血再灌注+LY294002组(I/R+LY组)。结扎冠状动脉前降支30min,再灌注120min。于缺血前、缺血30min以及再灌注60、90、120min时,测定左室收缩压(Lef tventricular systolic pressure,LVSP)、左心室舒张末压(Left ventricular end-diastolic pressure,LVEDP)、左室内压变化最大上升和下降速率(Maximum rate of left ventricular pressure development and decay,±dp/dtmax);测定心肌梗死面积百分比、一氧化氮(Nitric oxide,NO)和一氧化氮合酶(Nitric-oxide synthase,NOS)的含量。结果:I/R组较SH组各时间点的收缩及舒张功能显著降低,心肌梗死面积增加,NO和NOS含量明显降低;与I/R组比较,PU组各时间点的收缩及舒张功能下降程度明显减轻,心肌梗死面积明显减少,NO、NOS含量明显增加;PU+LY组与PU组相比各时点的收缩及舒张功能显著降低,心肌梗死面积增加,NO和NOS含量明显降低;PU+LY组、I/R+LY组与I/R组相比以上指标差异均无显著意义。结论:葛根素预处理对缺血再灌注大鼠心肌有较好的保护作用,其作用机制可能与PI3K/Akt信号通路的活化有关。Objective： To investigate the protective effect of preconditioning with Puerarin on myocardial ischemia reperfusion in adult SD rats and its possible mechanism. Methods： Forty Male SD rats were divided randomly into five groups （n=8）. The myocardial injury of rats ware produced by the ligation of left coronary artery for 30 min followed by 120 min reperfusion. LVSP, LVEDP, and ± dp/dtmax were recorded at ischemia 30 min, reperfusion 60, 90 and 120 min. The myocardial infarct size and the level of NO and NOS were observed. Results： In group I/R, the systolic and diastolic function were significantly reduced ; myocardial infarct size was increased ; NO and NOS were significantly lowered compared with those in group SH（P〈0.05 ）.The systolic and diastolic function were improved;myocardial infarct size was significantly smaller; the level of NO and NOS were increased significantly in group PU as compared with those in group I/R（P〈0.05 ）. All these parameters in group PU＋LY were not improved compared with those in group PU（P〈0.05 ）. There was no significant difference among the parameters of groups PU＋LY, I/R＋LY,and I/R. Conclusion： Preconditioning with puerarin can significantly reduce myocardial ischemia-reperfusion injury, the mechanism of which is partly via PI3K-Akt signal transduction.

关 键 词：葛根素 心肌 缺血再灌注 磷脂酰肌醇-3激酶 丝(苏)氨酸激酶 

分 类 号：R541.4[医药卫生—心血管疾病]