微囊微环境对脐血细胞造血相关基因表达的影响  

Influence of microcapsule microenvironment on hematopoietic gene expression of cord blood cells

作　　者：李双月[1,2] 王为[1] 王秋艳[3] 孙志杰[1] 吕国军[1] 郭昕[1] 朴丰源[2] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医学材料工程组,辽宁大连116023 [2]大连医科大学劳动卫生与环境卫生学教研室,辽宁大连116044 [3]辽宁出入境检验检疫局,辽宁大连116001

出　　处：《大连医科大学学报》2009年第6期625-628,共4页Journal of Dalian Medical University

基　　金：国家自然科学基金重点项目(20736006);国家卫生部传染病重大专项课题(2008ZX10002-019)

摘　　要：[目的]利用基因芯片技术研究微囊微环境对脐血细胞造血相关基因表达的影响。[方法]将新鲜分离的人脐血细胞在生理条件下进行微囊化包封、培养7 d后,对其进行总RNA提取、纯化、体外转录合成cRNA探针、cRNA探针生物素标记、与基因芯片杂交、扫描杂交信号,最后进行芯片数据处理和生物学信息分析。[结果]基因芯片筛选结果显示,与平面培养对照组相比,微囊化培养脐血细胞中差异表达2倍及以上的造血相关基因有32个,其中19个表达上调,13个表达下调。[结论]微囊微环境影响脐血细胞造血相关基因的表达。[ Objective ] To Study the influence of microcapsule microenvironment on the hematopoietic gene expression of cord blood cells. [ Methods ] Cord blood cells were cultured in microcapsules for 7 days, then the encapsulated cells were collected. Total RNA of collected cord blood cells was extracted, purified, and transcribed to cRNA probe. Then, cRNA probe was biotin - labeled and hybridized with gene chip. Hybridization signals were scanned and gene chip data was analyzed. [ Results] The differential expressions of a total 32 genes were found in microencapsulated cord blood cells compared with control group,including 19 up-regulated genes and 13 down-regulated genes. [ Conclusion ] Microcapsule microenvi-ronment may affect hematopoietic gene expression of cord blood cells.

关 键 词：微囊微环境 脐血细胞 基因表达 培养环境 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]