自组装肽包裹嗅神经鞘细胞抑制脊髓损伤后胶质瘢痕形成并促进轴突再生  

Self-assembling peptide-enwrapped olfactory ensheathing cells inhibit glial scar formation and promote axon regeneration after spinal cord injury

作　　者：王春婷[1] 贺诗华[2] 游思维[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,陕西西安710032 [2]西安科技大学化学与化工学院

出　　处：《解剖学研究》2009年第6期401-405,共5页Anatomy Research

基　　金：军队"十一五"专项(08Z028);军事医学重大项目(08XJZ002);军队科技攻关重点项目(06G089)

摘　　要：目的研究自组装肽包裹嗅神经鞘细胞(OECs)移植对大鼠脊髓损伤(SCI)后轴突再生的影响。方法成年雄性SD大鼠40只,行脊髓T_(10)节段背侧横断术,动物随机分为4组,每组10只。SAP-OECs组于间隙内移植SAP包裹的OECs悬液,SAp、OECs对照组同法分别移植等量SAP及DF12-OECs悬液;单纯全切对照组不做处理。术后2或4周过量麻醉处死动物,脊髓损伤节段连续冰冻矢状切片。行抗GAP-43和GFAP免疫组织化学和免疫荧光双标染色。结果SAP-0ECs组损伤区内有大量GAP-43免疫反应纤维,SAP组相较OECs及单纯半切对照组则可见相当数量GAP-43免疫反应纤维。OECs及单纯半切对照组损伤区周围可见一条致密度的GFAP阳性的反应性星形胶质细胞条带,而SAP组GFAP免疫反应纤维比对照组少,且多于SAP-OECs组。此外激光共聚焦显微镜下观察SAP-OECs组在损伤后4周GAP-43免疫反应纤维能通过位于脊髓损伤区尾端的GFAP阳性区域。结论自组装肽包裹嗅鞘细胞可以抑制脊髓损伤后胶质疤痕形成并促进轴突再生。Objective To investigate the effects of self-assembly peptide （SAP） gel-enwrapped olfactory ensheathing cells （OECs） transplantation on the axonal regeneration after the spinal cord injury in rats. Methods Forty adult Sprague-Dawley rats underwent spinal cord dorsal transection at T10 and were divided into 4 groups. Ten animals received intra-slit transplantation of Hoechst-labeled OECs enwrapped in SAP （SAP-OECs group）; the other animals served as control with similar transplantation of the same volume of SAP, DF12-OECs or nothing, respectively （SAP, OECs and lesion-control）. All animals were killed after a survival period of 2 or 4 weeks. The lesioned segments were removed, sectioned longitudinally, stained with antibodies against growth-associated protein-43 （GAP-43） and GFAP through immunohistochemical and double immunofluorescence technique. Results Numerous GAP-43-positive fibers were detected in the lesion epicenter of the scaffold plus cells cords which were within the space of the primary injury. Compared with OECs and lesion-control group, there were quite a few GAP-43- immunoreactive fibers in the SAP-alone cords. The lesion-control and cells alone cords formed a dense zone of GFAP-positive reactive astrocytes in the spinal cord surrounding the lesion site than the scaffold plus cells group. Immunostaining for GFAP in the scaffold-alone and lesion controls exhibited a smaller population of astrocytes in the injury edge of the former than the latter. Furthermore, GAP-43-positive axons could be clearly seen entering the region with GFAP-positive astrocytes in the spinal cord caudal to the injury. Conclusion These results may provide new insight into the ability of OECs to promote axonal regeneration and indicate a novel approach to SCI.

关 键 词：嗅神经鞘细胞 脊髓损伤 自组装肽 轴突再生 

分 类 号：R651.2[医药卫生—外科学]