紫杉醇预处理对猪体外成熟卵母细胞玻璃化冷冻的影响  

Effects of Pre-Treatment with Taxol on Vitrification of in Vitro-Matured Porcine Oocytes

作　　者：王磊[1,2,3] 杨山亭[1,2,3] 戴建军[1,2] 吴彩凤[1,2] 张廷宇[1,2] 张德福[1,2] 

机构地区：[1]上海农业科学院畜牧兽医研究所,上海201106 [2]上海农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]南京农业大学动物科技学院,南京210095

出　　处：《畜牧兽医学报》2009年第12期1817-1822,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：上海市科技兴农重点攻关项目(2005-3-5);上海市科技兴农推广项目(2007-3-7);上海市农科院青年科技发展基金(2008(07);2009(15));上海市农业遗传育种重点实验室开放课题(Shagb2008-03)

摘　　要：为了提高猪成熟卵母细胞细胞玻璃化冷冻效果。本试验拟添加紫杉醇,比较其对冷冻环冷冻效果的影响。结果显示使用1.0μmol.L-1紫杉醇预处理卵母细胞,冷冻后其形态完整率(89.93%)和FDA染色存活率(83.33%)都显著高于未处理组的79.12%和70.97%(P<0.05),继续提高紫杉醇浓度则表现为对卵母细胞的毒副作用。在不同预处理时间上,预处理30 min组冷冻后卵母细胞形态完整率和FDA染色存活率最高,分别达到90.21%和84.13%。预处理浓度1.0μmol.L-1,30 min是比较合适的处理方法;紫杉醇、细胞松弛素B(CB)或两者联合添加能显著提高猪成熟卵母细胞的玻璃化冷冻的效果(P<0.05),但两者之间没有显著差异(P>0.05)。The aim of this study was to improve the effect of vitrification of porcine matured oocyte.This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by a cryoloop method.The results showed that the vitrified oocytes pre-treated with 1.0 μmol·L-1 Taxol attained higher normal morphology rate（89.93%） and FDA stain survival rate（83.33%） than the control（79.12% and 70.97%）（P〈0.05）.But when the concentration of Taxol was increased,it came to weaken the effect of vitrification.The proportions of oocytes with normal morphology and survive rates could reach to 90.21% and 84.13%,when they were treated for 30 minutes before vitrification.So the best parameter was 1.0 μmol·L-1 Taxol for 30 minutes before vitrification.Pre-treatment with CB or Taxol had a significant positive effect on vitrification of porcine matured oocytes（P〈0.05）,but there were no significant differences between the two chemical materials（P〉0.05）.

关 键 词：猪 卵母细胞 冷冻环玻璃化冷冻 紫杉醇 

分 类 号：S814.8[农业科学—畜牧学]