S-腺苷甲硫氨酸脱羧酶基因沉默抑制乳腺癌MCF-7细胞生长的研究  

Gene silencing of S-adenosylmethionine decarboylase inhibits growth of breast cancer MCF-7 cells

作　　者：辛佳璇[1] 陈世敏[1,2] 王伟[1] 阎云飞[1] 徐春晓[1] 刘斌[1] 刘贤锡[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,济南250012 [2]济南军区总医院实验诊断科,济南250031

出　　处：《山东大学学报（医学版）》2009年第12期66-69,73,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关项目(2008GG30002030)

摘　　要：目的探讨S-腺苷甲硫氨酸脱羧酶(SAMDC)基因沉默对乳腺癌MCF-7细胞增殖及侵袭能力的抑制作用。方法用特异性沉默SAMDC基因表达的RNA干扰真核表达载体SAMDCshRNA表达载体(pGPU6-SAMDC)转染乳腺癌MCF-7细胞,采用RT-PCR和Western-blot技术分别检测SAMDC mRNA和蛋白质表达水平;细胞增殖实验(CCK-8法)和细胞周期分析评价SAMDC基因沉默对乳腺癌MCF-7细胞增殖的影响,肿瘤侵袭实验观察SAMDC基因沉默对乳腺癌细胞MCF-7侵袭活性的影响。结果SAMDCsh RNA表达载体转染后,MCF-7细胞SAMDC mRNA水平下调43.8%,蛋白表达水平下降66.5%;MCF-7细胞的增殖活性和侵袭能力均受到明显抑制,细胞增殖活性下降37%,细胞周期分析G1期细胞增加。结论SAMDC基因沉默可通过延长细胞周期的G1期而抑制乳腺癌MCF-7细胞的增殖,并能降低癌细胞侵袭活性,提示SAMDC可作为乳腺癌基因治疗的靶分子。Objective To investigate the inhibitory effect of the S-adenosylmethionine decarboylase（SAMDC）gene silencing on proliferation and invasion of breast cancer MCF-7 cells.Methods Breast cancer MCF-7 cells were transfected with a short hairpin RNA eukaryotic expression vector against SAMDC（pGPU6-SAMDC）.The effect of suppressing proliferation of MCF-7 cells was detected by cell proliferation assay（CCK-8）and cell cycle analysis.Semi-quantitative RT-PCR analysis of mRNA and western blot analysis of proteins were used to determine expression of the SAMDC gene.The invasive ability of breast cancer MCF-7 cells was assessed by invasion assay in vitro.Results After the shRNA expression vector was transfected into breast cancer MCF-7 cells,the growth and invasive ability of MCF-7cells were significantly inhibited,and the proliferation of cells was decreased by 37%.Gene expression of SAMDC in MCF-7 cells was also significantly inhibited,of mRNA was inhibited by 43.8% and of protein was inhibited by 66.5%.Moreover,the proportion of MCF-7 cells in the G0/G1 phase was increased.Conclusion The shRNA of the SAMDC gene can effectively inhibit the proliferation and invasion of MCF-7 cells and is a potential target in gene therapy of breast cancer.

关 键 词：乳腺肿瘤 S-腺苷甲硫氨酸脱羧酶 RNA干扰 基因治疗 

分 类 号：Q784[生物学—分子生物学]