二水磷酸氢钙涂层镁锌合金体内降解和新骨形成的研究  

Degradation of dicalcium phosphate dihydrate-coated Mg-Zn alloy and bone formation in vivo

作　　者：陶海荣[1] 顾剑华[1] 何耀华[2] 张岩[2] 张绍翔[3] 宋阳[3] 李佳楠[3] 赵常利[2] 蒋垚[2] 张小农[3] 

机构地区：[1]上海交通大学医学院第三人民医院骨科,上海201900 [2]上海交通大学第六人民医院骨科,上海200233 [3]上海交通大学材料科学与工程学院,上海200240

出　　处：《上海交通大学学报（医学版）》2009年第12期1410-1414,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30772182);上海市卫生局基金(2007Y24)~~

摘　　要：目的研究二水磷酸氢钙涂层镁锌合金在体内的降解规律及其对新骨形成的影响。方法72只新西兰大白兔均于左侧股骨髁钻孔,随机植入二水磷酸氢钙涂层镁锌合金棒(实验组,n=24)或镁锌合金棒(镁锌合金对照组,n=24)或聚乳酸棒(聚乳酸对照组,n=24)。术前1d及术后第1天和第1、2、5、10周时,检测实验组和镁锌合金对照组动物血清Mg2+浓度。术后第3、6、12、18周时,植入处X线摄片检查;摄片后三组分别处死6只动物,采集并制备肝、肾组织标本,HE染色后观察组织病理学改变。术后18周时,行股骨髁组织切片HE染色和品红苦味酸染色,观察植入物降解情况并比较骨矿物质沉积率。结果二水磷酸氢钙涂层组与镁锌合金对照组术前及术后各时间点的血清Mg2+浓度比较,差异均无统计学意义(P>0.05)。术后3周时,X线摄片观察发现镁锌合金对照组植入物旁有气泡产生。三组肝、肾组织学检查均未见明显异常。与两对照组相比,实验组骨矿物质沉积率较高,植入物的降解速度较慢。结论二水磷酸氢钙涂层镁锌合金体内降解时间延长,生物相容性更优良。Objective To investigate the degradation of dicalcium phosphate dehydrate-coated Mg-Zn alloy in vivo and bone formation. Methods Left femoral condyles were drilled in 72 New Zealand rabbits, and were randomly divided into experiment group （n=24, implanted with dicalcium phosphate dehydrate-coated Mg-Zn alloy rods）, Mg-Zn alloy control group （n=24, implanted with Mg-Zn alloy rods） and poly-L-lactide acid rod group （n=24, implanted with poly-L-lactide acid rods）. Serum concentrations of Mg^2＋ were examined 1 d pre-operation, and 1 d, 1 week, 2 weeks, 5 weeks and 10 weeks post-operation in experiment group and Mg-Zn alloy control group. Operation sites were examined by X-rays at 3, 6, 12 and 18 weeks post-operation. After X-ray examination at each time point, 6 rabbits in each group were sacrificed, and subjected to histopathological observation of live and kidney tissues by HE staining. Tissues from condyles of femur were observed by HE staining and 2, 4, 6-trinitrophenol rosein staining, and mineral apposition rate of bone was calculated. Results There was no significant difference in the concentrations of serum Mg^2＋ at each time point between Mg-Zn alloy control group and experiment group （P〉0.05）. X-ray examination revealed gas emerged near the implants 3 weeks after surgery in Mg-Zn alloy control group. However, there was no obvious histological abnormality in liver and kidney tissues. The mineral apposition rate was higher and the degradation of material was lower in experiment group than those in the other two groups. Conclusion Dicalcium phosphate dehydrate-coated Mg-Zn alloy has a favourable biocompatibility, and degrades more slowly in vivo.

关 键 词：二水磷酸氢钙 涂层 镁锌合金 降解 骨形成 

分 类 号：R318.08[医药卫生—生物医学工程]