肺炎衣原体与突发性耳聋的相关性研究  

Correlation between Chlamydia pneumoniae and sudden sensorineural hearing loss

作　　者：敖华飞[1] 毛小慧[1] 郭竹英[1] 

机构地区：[1]上海交通大学医学院第三人民医院耳鼻咽喉-头颈外科,上海201900

出　　处：《上海交通大学学报（医学版）》2009年第12期1487-1490,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(54119551)~~

摘　　要：目的探讨肺炎衣原体(Cpn)感染与突发性耳聋(SSHL)之间的关系。方法收集SSHL患者120例(SSHL组),另选120名健康者为对照组。分离人外周血单核细胞(PBMC),用直接免疫荧光法(DIF)及PCR分别检测PBMC中Cpn特异性抗原(Cpn-Ag)和Cpn-DNA的表达;微量免疫荧光法检测抗Cpn抗体IgA、IgG和IgM。结果DIF法与PCR法的检测结果比较,差异无统计学意义(P>0.05)。两组代表急性感染的Cpn-Ag和Cpn-IgM的检出率比较,差异有统计学意义(P<0.01);两组代表慢性感染的Cpn-IgA和Cpn-IgG的检出率比较,差异有统计学意义(P<0.05)。结论SSHL患者Cpn感染的检出率显著升高,Cpn可能是引起SSHL的一种因素。Objective To explore the correlation between Chlamydia pneumoniae （Cpn） infection and sudden sensorineural hearing loss （SSHL）. Methods One hundred and twenty patients with SSHL were enrolled as SSHL group, and another 120 healthy subjects were served as control group. Human peripheral blood mononuclear cells （PBMCs） were isolated, and the expression of Cpn-specific antigen （Cpn-Ag） and Cpn-DNA in PBMCs was detected by direct immunofluorescence test and PCR, respectively. Specific antibodies （IgA, IgG and IgM） to Cpn were determined by microimmunofluorescence test.Results There was no significant difference in the positive findings between direct immunofluorescence test and PCR （P〉0.05）. There were significant differences in the positive rates of Cpn-Ag and Cpn-IgM for acute infection between these two groups （P〈0.01）, and there were significant differences in the positive rates of Cpn-IgA and Cpn-IgG for chronic infection between these two groups （P〈0.05）. Conclusion There is a significantly higher prevalence of Cpn in patients with SSHL. Cpn infection may be a possible cause for SSHL.

关 键 词：肺炎衣原体 突发性耳聋 微量免疫荧光 

分 类 号：R764.437[医药卫生—耳鼻咽喉科]