CD4^＋CD25^＋调节性T细胞对哮喘大鼠气道炎症的影响  

The effects of CD4^＋CD25^＋ regulatory T cells on the airway inflammation of asthmatic rats

作　　者：薛克营[1] 王成国[1] 程立[1] 杨中卫[1] 王正艳[1] 李威[1] 石明[1] 唐友勇[1] 

机构地区：[1]郧阳医学院附属东风医院呼吸内科,湖北十堰442008

出　　处：《中国医师杂志》2009年第12期1613-1616,共4页Journal of Chinese Physician

摘　　要：目的观察CD4^＋CD25^＋调节性T细胞（CD4^＋CD25^＋Treg）对哮喘大鼠气道炎症的影响。方法将卵白蛋白（OVA）免疫耐受大鼠CD4^＋CD25^＋Treg细胞过继转移给哮喘大鼠，然后观察支气管肺泡灌洗液（BALF）中细胞计数及分类，ELISA检测BALF中IL-4、IL-5和IFN-1及血清OVA特异性IgE含量，HE染色观察肺组织的病理改变。结果与哮喘组比较，过继转移CD4^＋CD25^＋Treg细胞后哮喘大鼠BALF中细胞总数、中性粒细胞和淋巴细胞百分率降低（P〈0．05），嗜酸性粒细胞（Eos）百分率明显降低（P〈0．01）；BALF中IL4和IL-5含量明显降低，IFN-1含量明显升高，血清OVA特异性IgE含量明显降低（P〈0．05）；气道炎症明显减轻。结论过继转移OVA免疫耐受大鼠CD4^＋CD25^＋Treg细胞可以明显抑制哮喘的慢性气道炎症。Objective To observe the effects of CD4^＋CD25^＋ regulatory T cells （ CD4^＋CD25^＋ Treg） on the airway inflammation of asthmatic rats. Methods CD4^＋CD25^＋ Treg of OVA- immune tolerance rats were transferred to asthmatic rats. Then hronchoalveolar lavage fluid （BALF） was collected, and cytology study was conducted. The IL-4, IL-5, IFN-γand OVA-specific serum IgE level in BALF were determined by ELISA. The lung tissue was obtained, and histological analysis was done through H.E. Results Total cells number, the percentage of lymphocytes and neutrophils in BALF, the IL-4 and IL-5 BALF levels and the OVA-specific serum IgE level of adoptive transfer group were decreased （ P 〈 0. 05 ） , and the percentage of eosinophils （Eos） was significantly lower than that of asthma group （ P 〈 0. 01 ）, while its BALF IFN-γ level was higher than that of asthma group（ P 〈 0. 05 ）. Compared with that of asthma group, peribronchiole inflam- matory of treated group was alleviated. Conclusion CD4^＋CD25^＋ Treg of OVA- immune tolerance rats transferred to asthmatic rats can significantly alleviate the airway inflammation of asthmatic rats.

关 键 词：CD4阳性T淋巴细胞 T淋巴细胞 辅助诱导 哮喘/病理生理学 白细胞介素4/代谢 白细胞介素5/代谢 干扰素Ⅱ型/代谢 

分 类 号：R5[医药卫生—内科学]