黄芪联合福辛普利对白蛋白诱导大鼠肾小管上皮细胞NF—κB活性及骨桥蛋白表达的影响  

Effect of atragalus membranaceus combined fosinopril on NF- κB activity and OPN expression in overload albumine stimulating proximal tubular cells

作　　者：罗昌霞[1] 屈振繁[2] 张建鄂[1] 张永[1] 

机构地区：[1]郧阳医学院附属十堰市太和医院肾内科,湖北十堰442000 [2]郧阳医学院附属十堰市太和医院普外Ⅱ科,湖北十堰442000

出　　处：《中国医师杂志》2009年第12期1626-1628,共3页Journal of Chinese Physician

摘　　要：目的探讨黄芪联合福辛普利对白蛋白诱导肾小管上皮细胞内NF-κB活性及骨桥蛋白表达的影响。方法培养的肾小管上皮细胞为空白对照；对照组加去脂的小牛血清白蛋白（BSA）20mg／ml共同培养；干预组先加福辛普利5μmol／L，1／2h后再加入BSA20mg／ml，黄芪组在干预组基础上加黄芪40μl/ml，1／2h后再加入BSA20mg／ml，凝胶电泳迁移率法（EMSA）法及RT-PCR检测12h、24h各组NF—κB活性及骨桥蛋白表达的变化。结果黄芪组在12h、24h均可明显抑制白蛋白诱导的肾小管上皮细胞内NF-κB活性及骨桥蛋白的表达，与空白、对照组及干预组比较差异有统计学意义（P〈0．05）。结论黄芪联合福辛普利可明显抑制白蛋白诱导的肾小管上皮细胞内NF—κB活性及骨桥蛋白的表达。Objective To explore whether or not the function of atragalus membranaccus combined fosinopril on decreasing NF- κB activity and OPN expression in overload albumin stimulating proximal tubuler cells. Methods Cultured cells without stimulation were used as placebo. Cultured cells were incubated with bovine serum albumin fat acid free （BSA） 20mg/ml as the control. Cultured cells were incubated with fosinopril for different hours as the treatment. Cultured cells were incubated with atragalus membranaceus combined fosinopfil for different hours as the atragalus membranaeeus group. ELISA and RT-PCR were used to observe NF - κB activity and OPN expression after cultured for 12h, 24h. Result Atragalus memhranaceus combined fosinopril rapidly decreased NFκB activity and OPN expression. Compared with the other groups, the difference was significant. Conclusion Aatragalus membranaceus combined fosinopril can obviously decrease NFκB activity and OPN expression in overload albumine stimulating proximal tubular cells.

关 键 词：黄芪△/治疗应用 福辛普利/治疗应用 白蛋白类/药理学 肾小管 NF—κB/代谢 唾液糖蛋白类/代谢 

分 类 号：R692[医药卫生—泌尿科学]