IL-10基因给药对BXSB狼疮小鼠发病的影响  

Experimental study on intramuscularly injection of eukaryotic expression vector pcDNA3 IL-10 on BXSB mice

作　　者：杨高云[1] 袁勇[2] 姜学义[1] 张颖妹[2] 马大龙[2] 李世荫[1] 

机构地区：[1]北京医科大学第三医院皮肤科,100083 [2]北京医科大学卫生部医学免疫学重点实验室

出　　处：《中国皮肤性病学杂志》1998年第4期197-199,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的：为了观察白细胞介素10（IL－10）在系统性红斑狼疮（SLE）发病中的作用。方法：通过基因工程方法构建了真核表达载体pcDNA3－IL－10，采用基因给药方式先后两次将质粒DNA直接肌注于3．5月龄雄性BXSB狼疮小鼠．同时给对照组小鼠注射等量空载体质粒pcDNA3。结果：发现IL－10在该鼠体内过表达可导致其血清ANA滴度、IL－6水平及肾脏IgG沉积明显增加，而尿蛋白和血清GPT水平及肾脏C3沉积无明显差异。结论：提示IL－10在SLE发病中可能具有病理性作用，阻止或减少IL－10的分泌及作用，也许有助于SLE的治疗。In order to study the role of IL-10 in SLE. Method We constructed eukaryotic expression vector pcDNA3 IL-10 and intramuscularly injected it in male BXSB mice. 16 of 3. 5 month old male BXSB mice were divided into two groups, one group was twice injected the pcDNA 3-IL-10 at dose of 100 μg per mouse at the interval of 20 days. Another group was injected plasmid of pcDNA3 at the same time as control.Results IL-10 encoding plasmid had harmful effects on murine SLE with increased serum ANA, IL-6 level and kidney IgG deposition, Whereas no difference of kidney C3 deposition and serum GPT level between the two groups.Conclusion Excessive production of IL-10 may play a pathogenic role in SLE. Blocking or reducing IL-10 secretion may contribute to SLE treatment.

关 键 词：小鼠 BXSB 系统性红斑狼疮 IL-10 基因 给药 

分 类 号：R593.241[医药卫生—内科学]