人工环境影响胶原诱导性关节炎的内分泌观察  

Study on the changes of Endocrinology about the Effects of Artificial Environments on Collagen induced Arthritis

作　　者：肖长虹[1,2,3] 李留洋[1,2,3] 顾为望 胡文[1,2,3] 袁进 谢卫兵 

机构地区：[1]第一军医大学珠江医院 [2]第一军医大学实验动物中心 [3]广州东方红印刷公司医务所

出　　处：《中国中医基础医学杂志》1998年第7期15-17,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：广东省自然科学基金

摘　　要：目的探讨人工风寒湿与风湿热环境影响大鼠胶原诱导性关节炎（ＣＩＡ）发病的机理。方法在ＣＩＡ的诱导阶段，给予风寒湿或风湿热刺激，连续观察大鼠关节炎症积分。免疫后第３０天取血，ＥＬＩＳＡ法检测血清胶原抗体水平，放免法检测血清雌二醇（Ｅ２）及三碘甲状腺原氨酸（Ｔ３）含量。结果１．风寒湿、风湿热均可显著提高雌性鼠的胶原抗体水平，但风寒湿显著降低雌性鼠关节炎症积分。２．寒湿显著升高雌性鼠血清Ｅ２及Ｔ３含量。３．雌性鼠风寒湿ＣＩＡ、风湿热ＣＩＡ及雄性鼠单纯ＣＩＡ组关节炎症积分与Ｅ２含量呈显著负相关。Objective:To probe the mechanism that artifical environments wind cold dampness(WCD) and wind dampness hot(WDH) affect the development of collagen induced arthritis(CIA) in rats.Methods:In the inducing period of CIA,the Wistar rats were stimulated with artificial WCD or WDH environments.Observe the arthritis scores continuously,collect bloods on the 30 th day after the injection of immunization.The serum levels of the anti collagen Ⅱ antibodies was detected with ELISA,the oestrogen(E2) and triiodothyronion(T3) was detected with radioimmune.Results:1.Both WCD and WDH increased the serum levels of anti collagen Ⅱantibodies in female rats significantly,but WCD decreased the arthritis scores significantly in female rats.2.WCD increased the serum level of E 2 and T 3 in female rats.3.In the groups of WCD CIA and WDH CIA of female rats,CIA of male rats,there were significant negative correlation between the level of E 2 and the arthritis scores.Conclusion:It maybe the important mechanism argued for the WCD decrease the arthritic scores in female rats that WCD promote the secreting of E 2.

关 键 词：胶原诱导性 关节炎 风寒湿 风湿热 雌二醇 

分 类 号：R274.943[医药卫生—中西医结合]