解脲脲原体垂直感染与新生小鼠肺炎关系的研究  

Study on the relationship between newborn mouse interstitial pneumonia and Ureaplasma urealyticum vertical infection

作　　者：吕杰[1,2] 李凤云[1,2] 唐素兰[1,2] 

机构地区：[1]蚌埠医学院微生物学教研室 [2]安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2009年第12期1057-1059,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究计划项目资助(2004kj274)

摘　　要：目的:建立妊娠期解脲脲原体(Ureaplasma urealyticum,UU)生殖道感染的小鼠模型,观察UU垂直感染与新生小鼠肺炎发生的关系。方法:采用血清4型UU标准菌株生殖道感染妊娠期孕鼠,同时设立对照组,通过病原学检查、病理学及免疫组化等方法观察妊娠期UU感染与新生小鼠肺炎发生之间的关系及垂直传播情况。结果:病理检查显示UU感染组新生小鼠肺部有间质性炎症发生,且脾有多核巨细胞融合的炎性样变;病原学检测显示新生小鼠肺、脾部有UU定植,356只实验组新生仔鼠肺、脾UU阳性检出率分别为71.36%和33.34%;378只对照组相关组织未分离培养出UU;免疫组化显示新生小鼠肺部有UU抗原,而对照组则无。结论:妊娠期UU感染可通过垂直传播方式影响胚胎发育,从而导致新生小鼠间质性肺炎的发生。Objective：To observe the relationship between newborn mice interstitial pneumonia and Ureaplasma urealyticum （UU） infection during the period of pregnancy of a vertical transmission animal model by genital infection with UU during the course of cyesis. Methods：Pregnant mice were infected by blood serum type 4 UU through vagina,interstitial pneumonia and settlement of UU in the newborn mice were observed by the methods of UU culture,pathology and immunohistochemical staining test;at the same time, control group was established. Results： Pathological examination showed that interstitial pneumonia occurred in the newborn mice of experimental group and its spleen had multinucleated giant cell, the lung and spleen of newborn mice had field planting of UU by etiology examination. In experimental group, UU positive detection rates in 356 neonatal mice lung and spleen were 33.34% and 71.36% , respectively;while UU were not detected in cultures from 378 above-mentioned tissues in control group; there were also plenty of UU antigen in the lung and spleen of newborn mice by immunohistochemical staining test; while control group had no phenomenon. Conclusions：The experiment demonstrates that the infection of UU during the period of pregnancy could influence embryonic development through vertical infection and result in newborn interstitial pneumonia.

关 键 词：解脲脲原体 新生小鼠 胚胎发育 垂直感染 间质性肺炎 

分 类 号：R375[医药卫生—病原生物学]