短链脂肪酸作用下伤寒沙门菌对巨噬细胞凋亡的影响  

Macrophage apoptosis induced by short-chain fatty acid stressed Salmonella typhi

作　　者：刘婷婷[1] 马丽娜[1] 李凤云[1] 樊蓉[2] 

机构地区：[1]蚌埠医学院病原生物学教研室,安徽蚌埠233030 [2]蚌埠医学院第一附属医院供料室,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2009年第12期1060-1062,共3页Journal of Bengbu Medical College

基　　金：安徽省感染与免疫重点实验室项目基金资助(2004sys008);蚌埠医学院科研项目(BY0808)

摘　　要：目的:了解伤寒沙门菌及其短链脂肪酸(short-chain fatty acid,SCFA)作用菌诱导人巨噬细胞凋亡的差异。方法:使用流式细胞术检测SCFA作用菌及原菌诱导巨噬细胞2、4、8、12及24h的细胞凋亡率。结果:SCFA作用菌及原菌均能诱导巨噬细胞发生凋亡,与对照组比较差异均有统计学意义(P<0.01);SCFA作用组不同时间诱导细胞凋亡率均高于原菌组(P<0.01)。结论:SCFA可促进伤寒沙门菌诱导巨噬细胞凋亡。Objective：To compare the difference of the apoptosis of macrophage induced by Sahnonella typhi （S. typhi） and that treated by short-chain fatty acid（SCFA）. Methods ：The apoptosis ratio of macrophage at different times after infection with S. typhi or its SCFA forms were determined by flow cytometry. Results ： Both S. typhi and its SCFA forms induced apoptosis of the macrophage with significant difference in the rates of apoptosis compared with those of the control（ P 〈 0.01 ）. However, the rate of apoptosis induced by SCFA forms was obviously higher than that by S. typhi（ P 〈 0.01 ）. Conclusions： SCFA may promote the apoptosis of macrophage.

关 键 词：伤寒沙门菌 巨噬细胞 短链脂肪酸 细胞凋亡 

分 类 号：R378.23[医药卫生—病原生物学]