铜绿假单胞菌DHA-1型AmpC酶检测  

Detection of DHA-1 plasmid-mediated AmpC β-lactamase of Pseudomonas aeruginosa

作　　者：范德胜[1] 

机构地区：[1]淮北矿工总医院检验科,安徽淮北235000

出　　处：《蚌埠医学院学报》2009年第12期1123-1125,共3页Journal of Bengbu Medical College

摘　　要：目的：调查淮北矿工总医院临床分离铜绿假单胞菌（Pseudomonas aeruginosa,Pa）DHA群质粒头孢菌素β-内酰胺酶（AmpC酶）的产生情况。方法：收集2007-2008年36株临床多重耐药Pa,用K-B法检测其对12种临床常用抗生素的耐药性 用改良三维试验法检测耐药表型 用聚合酶链反应（PCR）扩增DHA结构基因。采用肠杆菌科基因间重复一致序列PCR（ERIC-PCR）方法对产DHA群Pa进行同源性分析。结果：36株临床多重耐药Pa中有25株扩增出DHA-1型酶基因,检出率为69.4%。25株Pa对10种抗生素的耐药率〉50%,ERIC-PCR分析结果显示在同一科室或不同科室间存在DHA-1流行。结论：淮北矿工总医院DHA-1型质粒AmpC酶检出率远高于国内其他地区 产DHA-1型质粒AmpC酶菌株对头孢菌素及单环酰胺类抗生素耐药严重,在同一科室或不同科室间的传播应引起高度重视。Objective：To investigate DHA plasmid-mediated AmpC β-lactamases in the clinical isolation of Pseudomonas aeruginosa（Pa）from patients in Huaibei Miner＇s General Hospital.Methods：Thirty-six Pa isolated from clinical specimens were collected and analyzed drug-resistance to clinical common antibiotics with K-B method,and detected the resistance phenotypes by modified three-dimensional test.After extraction of genomic DNA,DHA-1 gene was amplified by polymerase chain reaction（PCR）and sequenced.Homology analysis of these sequences was carried out by compared with DHA genes of enterobacteriaceae in gene bank.Results：DHA-1 gene was amplified by PCR in 69.4%（25/36）Pa isolations.The resistant rate of 25 Pa to 10 types of antibiotics was more than 50%,and ERIC-PCR analysis was shown DHA-1 was prevailed in the same or different departments in our hospital.Conclusions：The detection rate of DHA-1 is much higher than that in other regions;Pa isolated with DHA-1 gene showed powerful activities with resistance to cephalosporin and monocyclic lactam antibiotics.The prevalence of Pa in the same or different departments should be noted seriously.

关 键 词：假单胞菌 铜绿 β-内酰胺类 头孢菌素酶 质粒 微生物敏感试验 

分 类 号：R378.991[医药卫生—病原生物学]