黑龙江立克次体190KD蛋白基因的PCR/SSCP分析  

Genotypic Analysis of R.heilongjiang 190KD Protein Gene by PCR/SSCP

作　　者：陈雷[1] 吴益民[1] 刘昕昕[1] 魏安明[1] 胡玲美[1] 李忠义[1] 

机构地区：[1]沈阳军区军事医学研究所

出　　处：《中国公共卫生学报》1998年第4期214-215,共2页

摘　　要：应用斑点热立克次体１９０ＫＤ蛋白基因的ＰＣＲ／ＳＳＣＰ分析了我国斑点热群黑龙江立克次体分离株５４、３６的基因型，并与国际标准株和国内其它株作了比较。结果显示黑龙江立克次体呈现出独特的ＳＳＣＰ图谱，与国际标准株显著不同，也和国内其它分离株差异明显。而ＨＬ５４和ＨＬ３６株完全相同。基因单链分析说明，我国斑点热群立克次体分离株存在２个种，即西伯利亚立克次体种和黑龙江立克次体种。同时证明应用ＰＣＲ／ＳＳＣＰ分析斑点热群立克次体型别，方法筒便、省时。? A new molecular biological approach for the identification of Rickettsia is described This approach employs PCR of Rickettsia with primers located in the 190 KD protein gene flanking a variable region,and analysis of the amplified product was based on the principle of SingleStrand Conformation Polymorphism(SSCP).We tested 2 strains of R.heilongjiang,4 strains of Rickettsia.sibria isolated from North of China and the inference strains(R.rickettsiae and R.connrii).The SSCP pattern indicated that R.heilongjiang is a new strain of Spotted Fever Group.This technique is a simple and rapid method for the detection and identification of Rickettsia by PCR amplification with the use of conserved primers and identification by nondenaturing gel electrophoresis.

关 键 词：EFGR PCR SSCP 立克次体 基因分型 

分 类 号：R376[医药卫生—病原生物学]