用家蚕细胞表达HBeAg及其应用  

Hepatitis B Virus e Gene Expression in Bombyxmori Cells and Application of HBeAg

作　　者：李保同[1] 邓小昭[2] 乔仁良[2] 何亮 刁振宇[2] 高健 

机构地区：[1]全军医学检验中心 [2]南京军区军事医学研究所分子生物学研究室

出　　处：《中国公共卫生学报》1998年第4期219-220,共2页

摘　　要：对经重组病毒感染的ＢｍＮ细胞的表达产物ＨＢｅＡｇ进行实验研究，并与菌产ＨＢｅＡｇ进行了比较。结果表明用家蚕细胞表达的ＨＢｅＡｇ分别作为捕获和酶标抗原建立的检测抗ＨＢｅ方法，不仅传统方法检测的阳性标本全部阳性，而且在传统方法阴性（抗ＨＢｃ阳性）组中，检出２例阳性，在抗ＨＢｅ和抗ＨＢｃ全部阴性的４２例标本中，也发现２例阳性。说明本法的敏感性高于传统方法。同时，用此方法制备的ＨＢｅＡｇ，在相同的蛋白浓度下，其抗原反应性也比用原核细胞表达的ＨＢｅＡｇ高出１００倍，且与抗ＨＢｃ交叉反应低１００倍，表明用家蚕细胞表达的ＨＢｅＡｇ不但效价高，且特异性、敏感性均优于大肠杆菌表达系统产生的ＨＢｅＡｇ。? Hepatitis B Virus e Ag was expressed in Bombyxmori cells infected by recombinant virus,and was compared with HBeAg from E,coli.The results show that enzyme labelled HBeAg from BmN was wed to elect HBe antibody,there were all positive in positive samples,but 2 positive in negative samples which detected by eraditional methods,and 2 positive in 42 samples which were negative detected by HBcAb and HBeAb.Meanwhile,the results in Fig1 referred that in the same concentration,antigenicity of HBeAg from BmN was 100times as high as that from E.Coli.It demonstrate the HBeAg from BmN cells get higher titer and specificity than that from E.coli.

关 键 词：乙肝病毒E抗原 基因表达 家蚕细胞 应用 

分 类 号：R446.62[医药卫生—诊断学]