冷适应小鼠IEL和SPL表型测定和体外杀伤活性的实验研究  

Study on Phenotype Determination and Natural Cytotoxicity in Vitro of Murine Splenic and Intestinal Intraepithelial Lymphcytes in Cold Adaptation 

作　　者：尹旭辉[1,2,3] 杨成君[1,2,3] 杨晓临[1,2,3] 叶晓卉 周正任[1,2,3] 姜在福 

机构地区：[1]沈阳军区后勤部军事医学研究所 [2]中国医科大学微生物教研室 [3]沈阳军区总医院

出　　处：《中国公共卫生学报》1998年第4期232-234,共3页

摘　　要：为探讨冷适应时免疫细胞的作用，用ＣＤ３、ＴＣＲαβ、ＴＣＲγδ、ＣＤ４和ＣＤ８单克隆抗体对小鼠小肠上皮内淋巴细胞（ＩＥＬ）和脾淋巴细胞（ＳＰＬ）的表型及其体外的杀伤活性分别进行了测定。结果表明，暴寒５天，各表型细胞数量减少，ＣＤ３、ＴＣＲαβ和ＣＤ４细胞数明显下降，ＣＤ４／ＣＤ８倒置。暴寒１０天ＣＤ３、ＴＣＲαβ和ＣＤ４细胞数恢复正常，ＣＤ３、ＴＣＲγδ和ＣＤ８细胞数较高，ＴＣＲγδ细胞显著增加。ＮＫ活性与ＴＣＲγδＴ细胞数相关，ＴＣＲγδ细胞比例增多时，ＮＫ活性增高。提示暴寒初期对Ｔ细胞的数量和功能有一短时抑制，对ＴＣＲαβ较ＴＣＲＤγδ、ＣＤ４较ＣＤ８细胞抑制强。Phenotype determination and killer activity in vitro of murine intestinal intraepithelial lymphocytes (IEL) and splenic lymphocytes (SPL) were studied by using CD3、TCRαβ、TCRγδ、CD4、CD8monoclonal antibodies in order to explore the immune cellular effect in cold adaptation.The results showed that cell numbers of all phenotypes decreased after cold exposure for 5 days,CD3、TCRαβ and CD4 cell numbers were decreased significantly(P<001),CD4/CD8 was reversed.They were recovered to normal at the point of cold exposure for 10 days,CD3、TCRγδ和CD8 cell numbers were higher,TCRγδ cell number had a notable increased(P<005)。Natural killer activity was related to TCRγδ T cell number.The higher proportion of the TCRγδ T cell was,the higher natural killer activity of the TCRγδ T cell was.It was suggested that there was an inhibition of short time at the initial stage of cold exposure.The inhibition was stronger to TCRαβ,CD4 cells than to TCRγδ,CD8 cells.TCRγδ T cell had certain resistant ability to cold.

关 键 词：冷适应 T淋巴细胞青型 NK活性 实验 

分 类 号：R594.2[医药卫生—内科学]