京尼平对丝素蛋白交联的染色作用  

Cross-linking Staining of Fibroin by Genipin in Solution

作　　者：李令锦[1] 李俊嵩[1] 王东[1] 段晓琼[1] 丁克毅[2] 杜林方[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]西南民族大学化学与环保工程学院,成都610041

出　　处：《应用与环境生物学报》2009年第6期799-802,共4页Chinese Journal of Applied and Environmental Biology

基　　金：四川省科技厅项目(No.2008SG0025)资助~~

摘　　要：京尼平是一种环烯醚萜类化合物,能够与氨基酸反应生成蓝色素.制备的无色丝素蛋白溶液与京尼平溶液混合后,溶液显蓝紫色.利用紫外可见光光谱和荧光光谱,分析了整个反应过程,考察了温度、酸碱度等影响因素,并对颜色产物对光照的稳定性进行了分析,初步探讨了京尼平对丝素蛋白交联染色作用的机制.结果显示:生成的颜色产物在590nm处具有特征光吸收,高温和碱性条件有利于反应的进行;交联产物的颜色与反应时pH相关,酸性条件下偏蓝色,碱性条件下偏紫色;生成的颜色产物具有较高的稳定性,于自然光强下放置一个月或5000lx光强照射6h,590nm吸收值减小不显著.丝素蛋白的内源荧光显著降低,表明反应导致丝素蛋白构象的剧烈变化.京尼平可能与丝素蛋白中某些氨基酸侧链基团反应形成稳定的颜色产物.研究结果对环烯醚萜类化合物用于蚕丝染色具有一定的指导意义.Genipin,a natural iridoid compound,was transformed to blue pigment by the reaction with amino acids. Amethyst solution was formed when colorless fibroin abstracted from natural silk was mixed with genipin. The effects of temperature and acidity or alkalinity on genipin-fibroin reaction and the product stability against light were studied with UV-vis spectra analysis. The mechanism of the reaction was primarily investigated through fluorescence spetra. The results indicated that the reaction products had a characteristic absorbance at 590 nm, while high temperature and pH accelerated the color reaction and the color of the reaction products was correlated with pH changing, tending to be blue at acidic pH and purple at alkahne pH. The reaction products possessed a good stability against light because only a small decrease ofA590nm value was observed under natural light for one month or illumination at 5 000 lx for 6 h. The influorescence intensity of fibroin was obviously decreased after the reaction, suggesting that a drastic change of fibroin conformation happened. The results showed that genipin was cross-linked with some amino acid side chains of fibroin and produced a product with stable color. This study would be helpful for staining silk with natural iridoid compound. Fig 5, Tab I, Ref 18

关 键 词：京尼平 环烯醚萜 丝素蛋白 蚕丝 交联 稳定性 

分 类 号：Q503[生物学—生物化学]