制备双-[6-氧-(2-间羧基苯磺酰基-丁二酸-1,4-单酯-4)-]-β-环糊精手性HPCE柱分离药物  

Preparation Chiral HPCE Column of Bis[6-Oxygen-(2-m-Carboxylbenzene Sulfonyl-Amber Acid-1,4 Uniester-4)]-β-Cyclodextrin Derivative for Separation of Drugs

作　　者：沈静茹[1] 陈丽娟[1] 余学红[2] 林敏[1] 韦康[1] 

机构地区：[1]中南民族大学化学与材料科学学院国家民委分析化学重点实验室 [2]中南民族大学校医院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2009年第4期28-32,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省自然科学基金资助项目(95J60)

摘　　要：用自行合成的双-[6-氧-(2-间羧基苯磺酰基-丁二酸-1,4-单酯-4)]-β-环糊精衍生物制备高效毛细管电泳柱,以此β-环糊精衍生物作为电泳手性固定相分离利多卡因、普鲁卡因、布比卡因,得到了最佳电泳分离条件.与空管柱分离效果相比,利多卡因、普鲁卡因分离度有所改善,但未达到基线分离;布比卡因在4 min内实现了手性异构体的分离,分离度达到14.71.实验表明:卡因类手性药物与键合在柱上的β-环糊精衍生物有一定的相互作用,并对自制的键合β-环糊精衍生物高效毛细管电泳柱进行了初步的扫描电镜表征.A new chiral high performance capillary electrophoresis column of bis[6-oxygen-（2-m-carboxylbenzene sulfonyl-amber acid-1,4 uniester-4）]-β-CD was prepared.The new derivative was used as chiral stationary phase to separate lidocaine hydrochloride,procaine hydrochloride and bupivacaine hydrochloride by high performance capillary electrophoresis.Conditions of separation were optimized for lidocaine,procaine and bupivacaine.HPCE resolution（Rs） of lidocaine and procaine has higher than in void column.Baseline separation was not achieved.However,baseline separation of bupivacaine enantiomers was achieved within 4 min.The value of Rs is 14.71. There are interaction between the chiral drugs with β-Cyclodextrin derivative.A preliminary token of HPCE column bonded with bis[6-oxygen-（2-m-carboxylbenzene sulfonyl-amber acid-1,4 uniester-4）]-β-CD was indicated by at scanning electron microscope（SEM）×250.

关 键 词：Β-环糊精衍生物 高效毛细管电泳手性固定相 键合 利多卡因 普鲁卡因 布比卡因 

分 类 号：O657.8[理学—分析化学]