桔梗种质资源过氧化物同工酶分析  

Peroxide Isozyme Analysis of Platycodon grandiflorum(Jacq.) A.DC Germ Plasm

作　　者：郭靖[1] 王英平[1] 王志清[1] 艾军[1] 李昌禹[1] 郭崇民[1] 

机构地区：[1]中国农业科学院特特产研究所,吉林吉林132109

出　　处：《特产研究》2009年第4期25-27,共3页Special Wild Economic Animal and Plant Research

基　　金：国家科技基础工作平台项目(2003DEA3N032)

摘　　要：32份栽培桔梗种质过氧化物同工酶谱共显示17条不同的酶带,共呈现16个酶谱类型,其中,12个为对应种质的特征酶谱。每份种质有5～9条酶带,不同种质酶谱及活性存在一定的差异,其中,A1、B1、B2、B3等4条酶带为共有带,具有种的特异性;7条(A2、A3、A4、C2、C4、C5、D3)酶带为特异性酶带,其中白花桔梗有2条特征酶带(A2、C2)。为桔梗种质资源的收集、保存、遗传育种提供了一种新技术和方法。Seventeen bands were screened by separating peroxide isozyme spectrums of 32 cultivated germplasm resources of Platycodon grandiflorum（Jacq.）A.DC..These bands could be classified into 16 kinds of peroxide isozyme spectrums.Among these 16 kinds of peroxide isozyme spectrums,12 kinds were characteristic spectrum corresponding to its grem plasm.The number of peroxide isozyme spectrum bands of every germ plasm was spun from five to nine.Difference of spectrum bands and activity of Platycodon grandiflorum was found.There were four common spectrum bands named A1、B1、B2 and B3 which were characteristic bands of Platycodon grandiflorum.Besides,there were seven peroxide isozyme spectrums bands named A2、A3、A4、C2、C4、C5 and D3 which were specific bands.Among these seven specific bands,the A2、C2 are characteristic bands of white flower Platycodon grandiflorum.This study showed 16 kinds peroxide isozyme spectrum and 12 kinds peroxide isozyme spectrum were specific.

关 键 词：桔梗 过氧化物同功酶 酶谱 

分 类 号：S567.23[农业科学—中草药栽培] Q814.1[农业科学—作物学]