五味子根、藤茎及叶提取物中五味子甲素、乙素的含量测定  

Determination of Deoxyschizandrin and Schisandrin in Extracts from the Schisandra chinensis Baill Root,Rattan and Leaves

作　　者：魏忠宝[1] 孙成贺[2] 侯微[2] 王英平[2] 胡晶晶[3] 姜艳玲[1] 

机构地区：[1]长春中医药大学,吉林长春130017 [2]中国农业科学院特产研究所,吉林吉林132109 [3]中国药科大学,江苏南京210009

出　　处：《特产研究》2009年第4期56-58,共3页Special Wild Economic Animal and Plant Research

基　　金：科技部支撑计划(2007BAI38B03);吉林省科技厅项目(20075019)

摘　　要：目的采用HPLC法测定五味子根、藤茎及叶不同溶剂提取物中五味子甲素、乙素的含量,同时筛选出最佳提取溶剂,为扩大五味子的入药部位资源提供理论依据。方法采用Hypersil ODS2(4.6mm×250mm,5μm)色谱柱;流动相:A.甲醇、B.水,梯度洗脱,0～20min,70%～80%A、20～35min,80%～70%A;流速:1mL/min;检测波长:254nm。结果五味子根中五味子甲素、乙素的含量最高,乙醇为最佳提取溶剂。Objective To establish a HPLC method for the determination of deoxyschizandrin and schisandrin levels in extracts from the Schisandra chinensis roots,rattans and leaves using different solvents.Select the best extraction solvent.Provide a theoretical basis for widening resources of Schisandra chinensis.Method Determination was performed on a Hypersil ODS2 column（4.6 mm×250 mm,5 μm）.The mobile phase：methanol-water gradient elution with the flowrate of1 mLl/min.The wavelength was set at 254 nm.Results Determination of deoxyschizandrin and schisandrin levels in extracts from the Schisandra chinensis roots is the highest.Ethanol is the best extraction solvent.

关 键 词：HPLC 五味子 五味子根 五味子藤茎 五味子叶 

分 类 号：S567.23[农业科学—中草药栽培] R927.1[农业科学—作物学]