应用RAPD标记初步构建S_2×朝鲜洋梨遗传连锁图谱  

Construction of a genetic linkage map based on RAPD molecular markers in S_2×Chaoxianyangli

作　　者：巩艳明[1] 庄得凤[1] 曹后男[1] 徐影[1] 宗成文[1] 

机构地区：[1]延边大学农学院园艺系,吉林龙井133400

出　　处：《延边大学农学学报》2009年第4期229-237,共9页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金资助项目(30560091)

摘　　要：以S2、朝鲜洋梨及其F1杂种为试材,应用RAPD标记初步构建了S2×朝鲜洋梨遗传连锁图谱.结果表明,RAPD标记在S2×朝鲜洋梨杂种F1分离方式可分为3类,符合孟德尔分离的标记51个,占38.1%;偏孟德尔分离的标记13个,占9.7%;异常分离的标记3个,占2.2%.在符合1∶1或3∶1分离以及偏离孟德尔分离的64条标记中,平均每个引物产生2.2个作图标记,可用于构建两亲本和F1的遗传连锁图谱.采用32个孟德尔分离标记和7个偏分离标记构建S2×朝鲜洋梨遗传连锁图谱,共有15个连锁群,全长394.18 cM,标记间平均距离10.11 cM.S2,Chaoxianyangli and their F1 hybrids were used as materials to construct the genetic linkagemap of S2×Chaoxianyangli by RAPD markers. The results indicated that the segregation patterns of RAPD markers in S2× Chaoxianyangli F1 were divided into three types： the markers accorded with Mendelian segregation is 51, occupied 38.1%; deviated from Mendelian segregation markers is 13, the ratio is 9.7% ; abnomoral segregation markers is 3, the proportion is 2.2 %. The average of 2.2 available mapping markers per primer were yielded in the 64 hands which accorded with Medelian sergregation（1： 1 or 3 ： 1） and deviated from Medelian segregation . These markers can be used to construct the genetic linkage map of parents and F1. There were fifteen linkage groups in the S2× Chaoxianyangli linkage map which constructed by 32 Mendelian markers and seven distorted markers, it covered 394.18 cM, and the average distance was 10.11 cM .

关 键 词：分子标记 RAPD 遗传连锁图谱 

分 类 号：S661.2[农业科学—果树学] Q943.2[农业科学—园艺学]