NRSE/RE-1 dsRNA诱导小鼠胚胎干细胞分化为NSE阳性的神经元样细胞  

Effect of NRSE/RE-1 dsRNA in Inducing Differentiation of Mouse Embryonic Stem Cells into Neuron-like Cells

作　　者：李宏图[1,2] 顾文佳[1] 孟凡彪[1] 马兰兰[1] 庞希宁[1] 

机构地区：[1]中国医科大学发育生物学教研室,卫生部细胞生物学重点实验室,中国辽宁沈阳110001 [2]辽宁省计划生育科学研究院辽宁省生殖医学重点实验室,中国辽宁沈阳110031

出　　处：《生命科学研究》2009年第6期477-481,共5页Life Science Research

基　　金：辽宁省自然科学基金资助项目(20062090);辽宁省教育厅资助项目(2004D227)

摘　　要：应用电打孔的方法将化学合成的NRSE/RE-1d sRNA转染小鼠胚胎干细胞,在去除LIF的条件下,直接接种培养,观察其分化情况,并对分化结果进行相关检测.结果显示分化细胞呈明显的神经样改变,免疫荧光显示NSE阳性率为(82.3±8.1)%.说明通过转染NRSE/RE-1d sRNA能有效地诱导小鼠胚胎干细胞向神经元细胞分化.Mouse embryonic stem cells were transfected with NRSE / RE-1 dsRNA through the electropo-ration and then cultured in medium without LIF. The differentiated cells were observed and detected by related means. Their morphology were obviously neuron-like alteration and （82.3 ±8.1）% NSE-positive. These results showed that the exogenous NRSE / RE-1dsRNA can efficiently induce ESCs differentiate into neurons in vitro.

关 键 词：胚胎干细胞 NRSE/RE-1d SRNA 诱导分化 神经元样细胞 

分 类 号：Q254[生物学—细胞生物学]