不同浓度NaCl胁迫下盐芥与拟南芥有机渗透调节物质与抗氧化酶系统的比较  

Comparison of Osmolytes and Antioxidant Enzymes in Thellungiella halophila and Arabidopsis thaliana under NaCl Stress

作　　者：杨剑超[1] 袁芳[1] 王新慧[1] 史瑶瑶[1] 隋娜[1] 王宝山[1] 

机构地区：[1]山东师范大学生命科学学院/山东省植物逆境重点实验室,山东济南250014

出　　处：《山东农业科学》2009年第12期48-51,共4页Shandong Agricultural Sciences

基　　金：山东省博士后基金(200802009);国家高技术研究发展"863"计划资助项目(2007AA091701)

摘　　要：本试验检测了盐芥(Thellungiella halophila)和拟南芥(Arabidopsis thaliana)有机渗透调节物质脯氨酸和可溶性蛋白的含量,以及抗氧化系统中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)的活性。结果表明,高浓度NaCl(>100 mmol/L)处理后盐芥的渗透调节物质含量显著高于拟南芥。拟南芥抗氧化系统酶活性尤其是CAT活性,明显受制于高浓度NaCl环境(>100mmol/L),而盐芥的抗氧化系统并没有受到显著的不良影响。In this study, the following physiological parameters were measured both in Thellungiella halophila and Arabidopsis thaliana, including the contents of proline and soluble proteins both related to osmotic adjustment, and the activities of SOD, CAT, APX and POD. The results showed that, after the treatments with high concentrations of NaCl （ 〉 100 mmol/L）, the contents of osmotic adjustment substances in Thellungiella halophila were significantly higher than those in Arabidopsis thaliana. Furthermore, the activities of antioxidant enzymes, especially CAT activity,were dramatically inhibited by high - salt conditions （ 〉 100 mmol/L） in Arabidopsis thaliana, whereas they suffered no distinctly adverse effects under the same conditions in Thellungiella halophila.

关 键 词：盐芥 拟南芥 NACL胁迫 渗透调节物质 抗氧化酶系统 

分 类 号：Q949.748.3[生物学—植物学]