Efficiencies of Generating Selectable Marker-Free Transgenic Rice with Different Transformation Methods  

作　　者：Yu Heng-xiu Lu Mei-fang CHEN Xiu-hua GONG Zhi-yun LIU Qiao-quan Gu Ming-hong 

机构地区：[1]Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China

出　　处：《Rice science》2009年第4期254-260,共7页水稻科学（英文版）

基　　金：supported by the National Transgenic Research Project (Grant Nos. 2008ZX08001-006 and 2008ZX08010-002);the National Natural Science Foundation (Grant No. 30770131);the Program for New Century Excellent Talents in University (Grant No. NCET-07-0736);the Jiangsu Province Government (Grant Nos. BK2007510, 06KJA21018 and K05015) of China

摘　　要：To study the efficiency of generating selectable marker-free （SMF） transgenic rice, two transformation methods were employed for four rice varieties （Wuxiangjing 9, Longtefu, Xieqingzao and Zhenshan 97）. One method is by using a single twin T-DNA binary vector pYH592 in one Agrobacterium strain, which is composed of two separate T-DNA regions （one carrying an antisense Wx gene and the other carrying a HPTgene）. The other one, named as two-strain/two-vector system, is by using two separate binary vectors in two separate Agrobacterium cultures. The results indicated that the average co-transformation frequencies of the antisense Wx gene and the HPT gene were 10.1% and 45.0%, respectively, for the four rice varieties. And the SMF transgenic plants selected from the offsprings of co-transformants were 55.6% and 60.0% in the two-strain/two-vector and twin T-DNA vector binary systems, respectively.To study the efficiency of generating selectable marker-free （SMF） transgenic rice, two transformation methods were employed for four rice varieties （Wuxiangjing 9, Longtefu, Xieqingzao and Zhenshan 97）. One method is by using a single twin T-DNA binary vector pYH592 in one Agrobacterium strain, which is composed of two separate T-DNA regions （one carrying an antisense Wx gene and the other carrying a HPTgene）. The other one, named as two-strain/two-vector system, is by using two separate binary vectors in two separate Agrobacterium cultures. The results indicated that the average co-transformation frequencies of the antisense Wx gene and the HPT gene were 10.1% and 45.0%, respectively, for the four rice varieties. And the SMF transgenic plants selected from the offsprings of co-transformants were 55.6% and 60.0% in the two-strain/two-vector and twin T-DNA vector binary systems, respectively.

关 键 词：rice Agrobacterium tumeficien selectable marker-free CO-TRANSFORMATION 

分 类 号：S511[农业科学—作物学] Q943.2[生物学—植物学]