重离子辐照玉米种子引起的基因组DNA变异分析  

Analysis of Genomic DNA Variation by ~7Li and ^(12)C Heavy Ion Beams Irradiation on Maize(Zea mays L.)Seeds

作　　者：罗红兵[1] 孔福全[2] 倪嵋楠[2] 赵葵[2] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]中国原子能科学研究院,北京102413

出　　处：《激光生物学报》2009年第6期755-759,共5页Acta Laser Biology Sinica

基　　金：湖南省自然科学基金项目(04JJ3022;05JJ3034);湖南省教育厅项目(04C285);长沙市科技局重点项目(K0902151-61)

摘　　要：利用HI-13串列加速器产生的10 Gy、20 Gy、30 Gy和50 Gy^7Li和^(12)C重离子束对玉米自交系478的干种子进行辐照,并用RAPD分析技术分析其变异情况。结果表明重离子~7Li和^(12)C辐照玉米种子可引起当代基因组DNA水平上的变异,一定范围内变异频率与注入剂量有关,在相同剂量下~7Li离子辐照的诱变率高于^(12)C离子辐照。In order to analyze the genomic DNA variation with RAPD technique, the dry maize seeds of inbred line 478 were irradiated with 7Li and 12C heavy ions（ dose 10 Gy,20 Gy,30 Gy and 50 Gy ）which accelerated by HI-13 tandem accelerator. The main results are as follows： the irradiation of 7Li and 12C can induce genomic DNA variation. The variation percentages, within a certain limit, are related to irradiation dosage. At the same dosage, the mutagenetie percentage of 7Li was higher than that of 12C.

关 键 词：重离子 玉米 诱变育种 RAPD 

分 类 号：S513.35[农业科学—作物学]