皮暗斑螟性信息素研究  

Candidate Sex Pheromone Components of Persimmon Bark Borer,Euzophera batangensis

作　　者：温秀军[1] Blanka Kalinova 刘满光[3] Michal Hoskovec 

机构地区：[1]华南农业大学林学院,广州510642 [2]捷克科学院生物有机化学研究所,布拉格16610 [3]河北省林业科学研究院,石家庄050061

出　　处：《林业科学》2009年第12期83-89,共7页Scientia Silvae Sinicae

基　　金：中捷政府间科技合作项目(38-14);石家庄市科学技术发展计划项目(06752096A)的一部分;得到华南农业大学校长基金(4400-208012)的资助

摘　　要：通过室内人工饲养与枣树林间定时定点观测相结合的方法,对皮暗斑螟的生殖生物学进行观测。傍晚(17—20时)羽化的成虫占总量的45.71%,交尾多于午夜后至凌晨前进行。在室内通过气相色谱与触角电位测定相结合的方法,测定皮暗斑螟雄蛾对来自雌蛾性腺的不同化合物的反应,通过应用全二维气相色谱飞行时间质谱分析技术,初步明确了皮暗斑螟性信息素组分为(Z9,E12)-十四碳-9,12-二烯-1-醇[(Z,E)-9,12-14∶OH]和(Z9)-十四碳-9-烯-1醇(Z9-14∶OH)。对二组分及其同分异构体的单体和混合物进行了林间引诱试验,初步结果表明(Z,E)-9,12-14∶OH的诱虫效果较好。5,50,500和1000μg不同剂量诱芯的田间引诱试验证明,500μg剂量诱芯诱虫量最多。Persimmon bark borer,Euzophera batangensis,is a principal insect pest in jujube orchards.It may cause severe damage in jujube orchards and can also attack many other plants,such as loquat（Eriobotrya japonica）,Horsetail Tree（Casuarina equisetifolia）etc.The sex bionomic characteristics of the moth were observed and investigated in lab and in field.The emergence of the moth in evening（17：00-20：00）accounted for 45.71% of the total.The mating time mainly occurred from late night to early morning.Gas chromatography with electroantennographic detection（GC-EAD）detected responses of the male to different components in the female sex pheromone gland extract,and two-dimensional（GC×GC）gas chromatography with a time-of-flight mass spectrometric detector（TOFMS）tentatively determined the pheromone substances were（Z9,E12）-tetradeca-9,12-dien^-1-ol（（Z,E）-9,12^-14∶OH）and（Z9）-tetradec-9-en^-1-ol（9Z^-14∶OH）,and they were closely related to that from other Euzophera species.Spectral characteristics,retention behavior and the ability to elicit GC-EAD responses suggested that both the identified unsaturated alcohols were candidates of sex pheromone components for E.batangensis.Subsequently a field trapping test with other isomeric compounds of the（Z,E）-9,12^-14：OH and 9Z^-14：OH in jujube orchards showed that（Z,E）-9,12^-14：OH attracted significantly more male moth of the borer in comparison to control and others chemicals.For the different dosage（5,50,500,1 000 μg）response,field tests showed the dose of 500 μg（Z,E）-9,12^-14：OH trapped the moth of the borer most.

关 键 词：皮暗斑螟 性信息素 生殖生物学 枣树 

分 类 号：S767.33[农业科学—森林保护学]