尿毒症患者低渗血红蛋白释放试验的初步结果  

A preliminary study on hypotonic hemoglobin release test in patients with uremia

作　　者：高雅琼[1] 王彩丽[2] 高丽君[1] 苏燕[1] 秦良谊[1] 周立社[1] 秦文斌[1] 

机构地区：[1]包头医学院血红蛋白研究室,内蒙古包头014010 [2]包头医学院第一附属医院肾内科,内蒙古包头014010

出　　处：《临床和实验医学杂志》2010年第1期12-13,共2页Journal of Clinical and Experimental Medicine

基　　金：内蒙古自治区教育厅重点科研项目NJ09157

摘　　要：目的建立低渗血红蛋白定时释放试验,比较研究慢性肾衰(CRF)患者红细胞中血红蛋白的存在状态,探讨其机制和临床意义。方法用低渗血红蛋白释放试验(HRT)五管法比较研究CRF患者与正常人的血红蛋白释放情况。再用低渗HRT一管法比较研究5例CRF与4例非CRF患者的血红蛋白释放结果。结果与正常对照比较,CRF患者标本出现以下特点:白蛋白前移、血红蛋白A2稍前移、定时释放增多、原点残留和泄漏明显。5例CRF的血红蛋白释放强于4例非CRF患者。结论与正常人或非CRF患者比较,CRF患者血液成分的电泳释放行为变化较大。白蛋白前移可能是它与血中毒素结合有关、血红蛋白释放方面的变化也可能来自毒素对红细胞的影响。Objective To establish hypotonic hemoglobin release test（HRT） for comparative study of existing status of hemoglobin in RBC from normal controls and patients with uremia, and to explore its mechanism and clinical significance. Methods Hypotanic HRT with five tubes method was used for comparative study on results of hemoglobin release between normal persons and uremic patients. One tube method was used for comparative study in hemoglobin release results between non - uremic patients and uremic patients. Results In comparison with normal controls, samples of uremic patients showed following features ： antelocation of albumin, slight antelocation of HbA2, increased timed - release, obvious original remains and leakage. The HRT results of five patients with chronic renal failure （CRF） were stronger than those of patients with non - CRF. Conclusion The behavior of electrophoretic release of blood components from uremia is different from that of normal controls or non - uremic patients. Antelocation of albumin may be due to its binding with uremic toxin . Change in Hb release perhaps may be due to the effect of toxin on RBC membrane. But its mechanism in detail needs further investigation .

关 键 词：尿毒症 血红蛋白释放试验 低渗 红细胞 血浆 白蛋白 

分 类 号：R692.5[医药卫生—泌尿科学]