东北荷包猪FUT1基因多态性及其与产仔性能的关联分析  

Polymorphism of FUT1 Gene and Its Relationship with Litter Size in Northeast Hebao Pigs

作　　者：朱弘焱[1] 赵颂[1] 赵微[1] 苏玉虹[1] 

机构地区：[1]辽宁医学院,辽宁锦州121000

出　　处：《安徽农业科学》2010年第1期88-90,共3页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅重点实验室项目(2009S067)

摘　　要：[目的]分析荷包猪Fun基因多态性及其与产仔性能的关系，为荷包猪资源保存、品种选育和开发利用提供参考依据。[方法]提取猪耳组织DNA，采用PCR-RFLP技术分析荷包猪FE们的基因多态性，并分析其基因型与产仔数的相关关系。[结果]用胁丽I限制性内切酶对扩增片段进行酶切，检测到GG、AG和AA3种基因型。抗性纯合子AA型个体1～5各胎次产仔数均值均高于AG和GG型个体，且第3胎和第4胎的产仔数均值显著高于AG型和GG型个体。[结论]荷包猪Fun基因具有多态性，其基因频率与国外杜洛克基本一致，与国外长白、大白和两者杂交猪差别较大。AA基因型对产仔性能而言是有益基因型。[ Objective] To analyze the FUT1 gene polymorphism of Hebao pigs and its relationship with litter size and provide reference for conservation, breeding, development and utilization of Hebao pigs. [ Method] The porcine DNA was extracted from Hebao pigs＇ears, and the polymorphism of FUT1 gene was detected hy PCR - RFLP. Then the relationship between genotype and litter size was analyzed. [ Result] The FUT1 gene had three kinds of genotypes, GG, AG and AA, as indicated by digestion with Hin6 I . The average litter size from the 1^st parity to the 5^th parity was higher in the individuals with the genotype AA than in those with the genotype AG or GG. And this difference was significant in average litter size of the 3^rd parity and the 4^th parity （P 〈 0.05 ）. [ Conclusion ] The FUll gene was polymorphic in Hebao pigs, and the allele frequency is similar to that of foreign Duroc pigs but greatly different from that of foreign breeds such as Landraee, Large White, and Landraee x Large White pigs. The genotype AA is an effective genotype for litter trait.

关 键 词：荷包猪 FUT1基因 多态性 产仔性能 

分 类 号：S828.2[农业科学—畜牧学]