珠颈斑鸠线粒体12S rRNA基因的克隆·测序和分子进化分析  

Cloning,Sequencing and Molecular Evolution Analysis of Mitochondrial 12S rRNA Gene in Streptopelia chinensis

作　　者：王星果[1,2] 倪欢林[2] 范国伟[2] 卢祥云[2] 顾志良[2] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]常熟理工学院生物系,江苏常熟215500

出　　处：《安徽农业科学》2010年第2期611-613,638,共4页Journal of Anhui Agricultural Sciences

基　　金：2009江苏省高等学校大学生实践创新训练计划项目

摘　　要：[目的]获得珠颈斑鸠(Streptopelia chinensis)线粒体12S rRNA基因序列,并以此分析不同鸟类的分子进化关系。[方法]根据其他鸟类线粒体12S rRNA基因及侧翼序列设计引物,采用PCR的方法获得珠颈斑鸠线粒体12S rRNA基因片段,克隆后进行序列测定,利用Mega 4.0软件Neighbor-Joining法分析不同鸟类的进化关系。[结果]获得珠颈斑鸠线粒体12S rRNA基因序列长度为970 bp,碱基组成为:A=31.6%、C=28.9%、G=19.8%、T=19.7%,其中A+T含量高于G+C含量。通过与GenBank中环颈斑鸠(Streptopelia capicola)等其他8种鸟类12S rRNA基因序列比较发现,珠颈斑鸠与环颈斑鸠的同源性最高,为94.4%,与家鸭(Anas platyrhynchos)的同源性最低,为78.4%。并根据这9种鸟类序列用NJ法构建进化树,结果表明,珠颈斑鸠和其他8种鸟类在分子水平上反映的进化关系与在形态学上的进化关系基本吻合。[结论]首次得到珠颈斑鸠12S rRNA基因全长序列,并确定了珠颈斑鸠与其他8种鸟类的分子进化关系。[ Objective ] This research aimed to obtain the sequence of 12S rRNA gene of Streptopelia chinensis and reconstruct the phylogeny of 9 species of birds based on 12S rRNA sequence. [ Method] The primers were designed based on 12S rRNA gene and flank sequence of other species of birds. Fragments were amplified by PCR and cloned into plasmid,and then sequenced. The phylogenetic tree of 9 species of birds was reconstructed using NJ method by Mega 4. 0. [ Result ] The length of the obtained 12S rRNA gene of Streptopelia chinensis was 970 bp, the base composition was： A = 31.6% ,C = 28.9% ,G = 19.8% ,T = 19.7% ,respectively,and the A ＋ T content was higher than G ＋ C content. The 12S rRNA gene sequence of Streptopelia chinensis was aligned with Streptopelia capicola and other 7 species of birds on GenBank. The results showed that the sequence homology was highest at 94.4% between Streptopelia chinensis and Streptopelia capicola ,and lowest at 79.8% between Streptopelia chinensis and Arias platyrhynchos. A phylogenetic tree was constructed using the neighber-joining method based on 12S rRNA gene sequence. According to the map analysis ,the molecular phylogenetic relationships of Streptopelia chinensis with other eight species of birds were consistent with their morphologic phylogenetic relationships. [ Conclusion ] The entire sequence of 12S rRNA gene of Streptopelia chinensis was successfully obtained. A phylogeny of Streptopelia chinensis and other 8 species of birds was reconstructed.

关 键 词：珠颈斑鸠 线粒体12S RRNA基因 分子进化 

分 类 号：S188[农业科学—农业基础科学]