梨的试管微嫁接技术研究  

Study on Micro-grafting in vitro of Tube Pear

作　　者：赵巍巍[1] 陈蕾[1] 曹后男[1] 宗成文[2] 姜淑苓[3] 

机构地区：[1]延边大学农学院园艺系,吉林龙井133400 [2]南京农业大学园艺系,江苏南京210095 [3]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《安徽农业科学》2010年第2期624-626,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30560091;30960231)

摘　　要：[目的]研究试管梨苗嫁接的影响因素及较适合的条件。[方法]以苹果梨、香水梨和山梨种胚培养的组培苗为试材,研究不同的消毒时间、嫁接方法及生长调节剂等因素对其生长的影响。[结果]种子消毒处理及鲜果取种组培苗的成活率均较高,达75.0%以上;以香水梨和苹果梨为接穗,无根山梨为砧木时的嫁接苗成活率有显著差异,其中以香水梨为接穗的成活率较高;以香水梨为接穗,山梨和香水梨为砧木时,带根的材料嫁接时的成活率都显著高于无根嫁接苗的成活率;此外,添加植物生长调节物质可以有效提高嫁接成活率,在培养基MS+BA 1.0 mg/L+IBA 0.3 mg/L中嫁接苗的成活率最高,达86.7%。[结论]该研究可为梨无性系砧木的扩繁推广和作为基砧应用提供了理论依据。[ Objective ] The research aimed to study the influencing factor and adaptive levels of survival rate of grafting and rooting rate of tube pear. [ Method] The experiment adopted the test tube plants of Pingguoli, Xiangshuili and Shanli as materials ,and the effects of different disinfection time, micro - grafting methods and hormone on its growth were studied. [ Result ] The results showed that tissue culture seedlings cultured with disinfection seeds and fresh fruit seeds had higher survival rates ,which could reached above 75.0%. Taken Pingguoli and Xiangshuili for cions,Shauli without root for rootstock, the survival rate of graft seedlings had obvious difference. And Xiangshuili for cions had higher survival rates. Taken Xiangshuili for cions, Shanli and Xiangshuili for rootstock,the survival rate of graft seedlings with root had higher survival rates than that without root. Added hormone to the media could improve the survival rate of micro - grafting plants, in the medium of MS ＋ BA 1.0 mg/L ＋ IBA 0.3 mg/L,the survival rate reached 86.7%. [ Conclusion]This study could provide theoretical basis for the promotion of clonal rootstock of pear and the application as base stock.

关 键 词：梨 离体培养 微型嫁接 成活率 

分 类 号：S661.2[农业科学—果树学]