短时间不同剂量UV-B辐射处理对冬小麦幼苗生理指标的影响  

Influences of Different UV-B Radiation Treatments in Short Time on Some Physiological Characteristics of Winter Wheat Seedlings

作　　者：张文会[1] 王明卓[1] 苗秀莲[1] 张玉霞[1] 李宝娟[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《安徽农业科学》2010年第2期648-650,667,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30771156);聊城大学大学生科技创新项目(SRT08111SM2)

摘　　要：[目的]研究短时间不同剂量UV-B辐射对冬小麦幼苗叶片生理指标的影响,为进一步研究植物应答短时间紫外线照射机理提供参考。[方法]以冬小麦为试验材料,采用15和30μW/cm2的UV-B照射强度对冬小麦幼苗进行短时间照射处理,并测定色素含量、光合速率等生理指标。[结果]UV-B处理导致叶绿素、可溶性蛋白含量以及含水量都降低,且存在剂量效应,30μW/cm2处理的降幅大于15μW/cm2;UV-B辐射处理对类胡萝卜素含量的影响与花青素相同,均表现为先下降后上升的趋势,2种剂量间差异不大;2种剂量的UV-B处理均抑制了PSⅡ的电子传递,尤其是处理2 h的抑制作用最大,4、6和8 h的抑制作用有所缓解,且存在剂量效应;2种剂量的UV-B辐射均抑制了叶片的光合速率,随着照射时间的延长抑制效应加大;高剂量UV-B处理对膜系统的损伤较大,低剂量UV-B处理结果不明显。[结论]短时间UV-B辐射处理对冬小麦幼苗叶片的影响具有剂量效应,高剂量比低剂量的作用效果大。且随着处理时间的延长,受害程度有所减轻,该结果与长时间UV-B照射的结果不同。[ Objective ] Studying the influences of different UV-B radiation treatments in short time on some physiological characteristics of water wheat seedlings was to provide reference for the mechanism of plant response under ultraviolet irradiation in short time. [ Method] The winter wheat were taken as materials to radiate by 15 and 30μW/cm^2 UV-B ,then the physiological indexes such as pigment content and photosynthetic rate were determined. [ Results ] The UV-B treatment caused the declines of chlorophyll content,soluble protein content and water content of leaves,besides,dose-effect was existed. The decline of 30 μW/cm^2 treatment was bigger than that of 15 μW/cm2 treatment. The influences of UV-B radiation on carotenoid content and anthoeyanidin content were similar; the change trends were declined firstly then increased. The UV-B treatment with two doses restrained the electron transport of PS II , particularly ; the inhibitory effect was biggest after treated 2h, and then this effect was declined in 4,6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system ,while the result of low dose treatment was not obvious. [ Conclusion] UV-B radiation treatment had dose-effect on winter wheat seedlings in short time and the influence of high dose was bigger than that of low dose. With the increase of treatment time, the damage was alleviated ; besides, the result was not similar to that of UV-B radiation in long time.

关 键 词：UV-B辐射 冬小麦幼苗 生理指标 

分 类 号：S512.1[农业科学—作物学]