HPLC-ELSD法测定银杏酮酯胶囊中萜类内酯的含量  

Content Determination of Terpene Lactones in Yinxing Tongzhi Capsules by HPLC-ELSD

作　　者：刘艳[1] 王艳[1] 张玉萍[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《安徽中医学院学报》2009年第6期63-65,共3页Journal of Anhui Traditional Chinese Medical College

摘　　要：目的:建立银杏酮酯胶囊中萜类内酯含量的测定方法。方法:采用高效液相色谱法蒸发光散射检测器(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)。色谱柱为KromasilC18柱;四氢呋喃-甲醇-水(12:18:70)为流动相。ELSD检测参数:漂移管温度:101℃;载气:N2;流速:3.12L/min。结果:银杏酮酯胶囊中基质对萜类内酯的测定无干扰,银杏内酯浓度自然对数与色谱峰面积自然对数的线性关系良好,白果内酯、银杏内酯A、银杏内酯B、银杏内酯C的重现性试验RSD分别为1.09%、1.12%、1.21%、1.03%,萜类内酯的平均回收率为97.73%,RSD为1.44%。结论:本方法简便易行,可作为银杏酮酯胶囊中萜类内酯成分有效检测方法。Objective： To set up a method for content determination of terpene lactones in Yinxing Tongzhi Capsules, a prescription of ketoesters extracted from Ginkgo biloba. Methods. High performance liquid chromatography-evaporative light scattering detector was employed. Chromatographic column was t（romasil C18, the mobile phase consisted of tetrahydrofuran-methanol-water （12：18：70）. The operating conditions of ELSI） were as follows：Drift tube temperature was 101 ℃,the carrier gas was N2, the flowrate was 3. 12 L/rain. Results： The suhstrales in Yinxing Tongzhi Capsules had noninterference with the determination of terpene lactones. There was a good linear relationship between natural logarithm of bilobalide concentration and natural logarithm of chromatographic peak area. The relative standard deviation （RSD） of reproducibility test about bilobalide, ginkalide A, ginkalide B and ginkalide C was 1. 09%, 1.12%,1.21%,1.03%,respectively. The average recovery of terpenelactoneswas97.73%,andRSDwas 1.44%. Conclusion： This method is an effective method for the determination of terpene lactones in Yinxing Tongzhi Capsules because of its convenience and accuracy.

关 键 词：银杏酮酯胶囊 萜类内酯 高效液相色谱法 蒸发光散射检测器 

分 类 号：R927.2[医药卫生—药学]