甲醛致V79细胞DNA-蛋白质交联作用及其修复  

Effect of formaldehyde on DNA-protein crosslink and its repair in V79 cell line

作　　者：江中发[1] 胡建国 李宁[1] 张玲[1] 袁春[1] 张本延[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430065 [2]杭州经济开发区白杨街道社区卫生服务中心,浙江杭州310018

出　　处：《中国工业医学杂志》2009年第6期413-415,共3页Chinese Journal of Industrial Medicine

基　　金：国家科技支撑计划项目(2006BAI19B05-2)

摘　　要：目的探讨甲醛诱导DNA-蛋白质交联作用及其修复效应。方法采用培养V79细胞株作为实验材料,以不同浓度的液态甲醛(0、50、100、200、400、800、1 600μmol/L)对细胞染毒1 h后检测DPC率,并根据实验结果选取200μmol/L对培养细胞株进行修复实验(修复0、6、12、18、24 h)。采用KCl-SDS沉淀法检测DPC率。结果较高浓度的液态甲醛(≥200μmol/L)可以产生明显的DPC效应(P<0.05);由200μmol/L浓度的液态甲醛诱导V79细胞产生明显的DPC(P<0.05),经过修复可以恢复到空白对照水平(P>0.05)。结论较高浓度的甲醛可以明显诱导DPC形成,甲醛所致的DNA-蛋白质交联可以得到修复。Objective To explore the effect of formaldehyde（FA）on DNA-protein crosslink（DPC）and its repair.Methods V79 cell line from Chinese hamster was used in the study.The cells were cultured with different concentrations of FA solutions（0,50,100,200,400,800,1 600 μmol/L）for 1 h.DPC rate in V79 cell line was detected by KCl-SDS assay,the repair of DPC in 200 μmol/L FA solution was examined at 0,6,12,18 and 24 h respectively after exposure.Results The DPC in cells exposed to higher concentrations of FA solution（≥200 μmol/L）were significantly higher than those of control group（P〈0.05）,while the DPC induced by 200 μmol/L of FA solution could be repaired after 18 h and restored to control level after 24 h.Conclusions The results suggested that higher concentration of FA might induce DNA-protein crosslink,however this kind of DPC could be well repaired.

关 键 词：甲醛 V79细胞 DNA-蛋白质交联 修复 

分 类 号：R994.6[医药卫生—毒理学] O622.4[医药卫生—药学]