酶动力学模块软件求解米氏常数和最大反应速率的实验研究  

An Experiment Study of K_m and V_(amx) Solution Based on Enzyme Kinetics Module Software

作　　者：李丽[1] 施展[2,3] 钱春香[1] 

机构地区：[1]东南大学材料科学与工程学院绿色建材技术研究所,江苏南京211189 [2]东南大学材料科学与工程学院,江苏南京211189 [3]江苏博特新材料有限公司,江苏南京210008

出　　处：《洛阳理工学院学报（自然科学版）》2009年第4期1-3,15,共4页Journal of Luoyang Institute of Science and Technology：Natural Science Edition

基　　金：国家自然科学基金资助项目(50578035)

摘　　要：当前仍多用作图法求解酶动力学米氏方程中米氏常数和最大反应速率。本文介绍采用酶动力学模块求解米氏常数和最大反应速率的办法。该模块是社会科学统计软件包中一项专门针对酶动力学数据分析的软件。通过对比双倒数作图法和酶动力学模块法求解出的米氏常数和最大反应速率,发现虽然二者的结果非常接近,但后者比前者需要的步骤少,时间少,人工少。故将酶动力学分析模块推荐给科研工作者,以供快速处理大量酶动力学实验数据时使用。Linewaver-Burk Equation is mostly used to draw a figure of 1/v - 1/[S] to work out Km and Vmax presently. However this paper recommends a method by using Enzyme Kinetics Module software. EKM is a module of Social Sciences Statistical Package serving for enzyme kinetics data analysis. Though similar in results, EKM is obviously not only more efficient than the Linewaver-Burk equation method to work out Km and Vmax, but also simpler, faster and more energysaving. Thus it is strongly recommended in this article to the technology researchers to be used in efficient dealing with huge quantity of Enzyme Kinetics experiment data.

关 键 词：酶动力学模块 米氏常数 最大反应速率 米氏方程 双倒数方程 

分 类 号：O643.1[理学—物理化学]