低温冷冻对皮肤组织桥粒芯糖蛋白1表达影响的实验研究  

Expression of desmoglein 1 in cryopreserved human skin

作　　者：贾晓明[1] 李东杰[1] 马彩虹[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100048

出　　处：《军医进修学院学报》2010年第1期53-54,57,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目 (30070298;30672177);首都医学发展科研基金项目 (2005-3087)~~

摘　　要：目的观察桥粒芯糖蛋白1(Desmoglein1,Dsg1)在不同温度保存皮肤组织中的分布,以探讨其与皮肤冷冻损伤的关系。方法用Dsg1抗体对5种保存条件下的皮肤组织作免疫组化染色,采用计算机图像定量分析方法测定表皮组织中的含量,并用H.E染色观察皮肤的组织结构变化。结果与新鲜皮片组相比,-196℃条件下皮肤组织结构保存较好,Dsg1含量变化不明显,而4℃、-20℃、-80℃保存皮肤组织结构破坏明显,含量亦明显下降。结论皮肤低温保存冷冻损伤可能与桥粒跨膜蛋白Dsg1破坏有关。Objective To observe the distribution of desmoglein l（Dsg 1） in cryopreserved human skin and to investigate its relation with skin cryoinjury. Methods Skin tissues cryopreserved under 5 different conditions were stained with immunohistochemistry using antibodies of dsg 1. Computer image quantitative analysis was used to measure DSG 1 level in epidermal tissues. Changes in structure of epidermal tissue were observed with H＆E staining. Results Compared to the normal human skin, no significant change was found in the histological structure of and desmogleinl level in human skin preserved at -196℃ . However, significant destruction and decreased Dsg 1 level were observed in human skin preserved at 4℃ , -20℃ , and -80℃ . Conclusion Skin cryoinjury may be correlated with the destruction of desmoglein 1.

关 键 词：皮肤 桥粒芯糖蛋白1 低温保存 

分 类 号：R329.499[医药卫生—人体解剖和组织胚胎学]