活性真皮基质的研制及生物学活性检测  

Construction of living dermal matrix and detection of its bioactivity

作　　者：马忠锋[1] 柴家科[2] 杨红明[2] 梁黎明[2] 许明火[2] 

机构地区：[1]河北省秦皇岛市第一医院外科,河北秦皇岛066000 [2]解放军总医院第304临床部烧伤整形科全军烧伤研究所,北京100048

出　　处：《军医进修学院学报》2010年第1期61-62,66,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：首都医学发展科研基金 (2005-2040);北京市科技计划研发攻关课题 (H060920050830);河北省科学技术研究与发展指导计划项目 (072761594);全军医学科研"十一五"计划专项课题 (06Z054)~~

摘　　要：目的构建含有成纤维细胞(Fb)-胶原-猪去细胞真皮基质(PADM)的活性真皮基质。研究培养液中转化生长因子-β1(TGF-β1)和碱性成纤维细胞生长因子(bFGF)的分泌情况。方法将Fb与胶原混合种植于PADM的表面,收集第2、4、6、8、10d培养液样本,双抗体夹心ELISA法测定其中TGF-β1、bFGF的含量。结果Fb在胶原内结构完整,与PADM形成复合真皮基质。TGF-β1和bFGF浓度培养第4天与第2天比较显著上升(P<0.01),并达到稳定水平。结论Fb-胶原-PADM真皮替代物可作为构建组织工程全层皮肤的真皮支架。Objective To construct the living dermal matrix containing fibroblasts-collagen-PADM and to investigate the secretion of TGF-β1 and bFGF in culture medium. Methods Fibroblasts were mixed with collagen inoculated on the surface of PADM. Culture medium samples were collected after 2, 4, 6, 8, and 10 days. TGF-β1 and bFGF levels in the culture medium were measured by ELISA. Results The structure of fibroblasts located in collagen was intact. Composite dermal matrix was constructed with PADM. The TGF-β1 and bFGF levels were higher on day 4 than on day 2 （P〈0.01） and then remained stable. Conclusion Living dermal matrix containing fibroblasts-collagen-PADM can be used as a substrate for constructing dermal skin in tissue engineering.

关 键 词：成纤维细胞 胶原 猪去细胞真皮基质 活性真皮基质 细胞因子 

分 类 号：R392.4[医药卫生—免疫学] R394.8[医药卫生—基础医学]