微球化骺板细胞构建兔异位骺软骨实验  

Construction of epiphyseal cartilage in rabbits with microencapsulated epiphyseal plate chondrocytes

作　　者：李文超[1] 许瑞江[1] 黄靖香[2] 卢强[2] 张莉[2] 赵斌[2] 聂少波[2] 

机构地区：[1]解放军总医院小儿外科,北京100853 [2]解放军总医院骨科,北京100853

出　　处：《军医进修学院学报》2010年第2期164-166,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金(30772276);解放军总医院科技创新课题(06ZS02)~~

摘　　要：目的探讨海藻酸钙-多聚赖氨酸微球复合骺板细胞,在兔体内异位构建组织工程化骺软骨的可行性。方法将兔异体骺板软骨细胞接种于海藻酸钙-赖氨酸凝胶微球中,体外培养后植入兔背部皮下,分别于2、4、8周取材进行大体及组织学观察。结果体外培养时微球中骺板细胞生长、增殖情况良好;移植皮下后第4周取材可见外形呈类骺软骨样组织块,行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色,镜下观察可见大量软骨细胞及类软骨陷窝样结构,植入的海藻酸钙微球周围组织无明显炎症反应。结论海藻酸钙-赖氨酸具有良好的组织相容性,复合异体骺板细胞形成微球,植入同种异体动物体内可以构建类骺软骨样组织。Objective To investigate the possibility to construct of ectopic tissue-engineered epiphyseal cartilage in rabbits with calcium alginate and polylysine microencapsulated chondrocytes. Methods Rabbit ectopic chondrocytes were inoculated into calcium alginate and polylysine gel microspheres. After the microspheres were cultured, they were implanted into the rabbit dorsal subcutaneous tissue. Samples were taken after 2, 4, and 8 weeks, respectively, for gross histological observation. Results The chondrocyes in microspheres grew and proliferated quite well during in vitro culture. A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagen Ⅱ . A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres. Conclusion Calcium alginate and polylysine microspheres have a rather good histocompatibility and can be used to construct epiphysis plate-like cartilage tissues by implanting them into homologuous animals.

关 键 词：生长面 组织工程 钙 藻酸盐 赖氨酸 

分 类 号：R68[医药卫生—骨科学] R684.103[医药卫生—外科学]