好氧反硝化菌筛选及强化OGO反应器脱氮的研究  

Screening of Aerobic Denitrifying Bacteria and Enhancement of Nitrogen Removal in OGO Reactor

作　　者：罗固源[1] 汤丽娟[1] 许晓毅[1] 林晓东[1] 王图锦[1] 陈燕云[1] 

机构地区：[1]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《中国给水排水》2010年第1期16-19,共4页China Water & Wastewater

基　　金：科技部国际合作项目(2007DFA90660);重庆市科技攻关项目(CSTC;2006AA7003;2008BB7305)

摘　　要：从具有同步硝化反硝化(SND)现象的OGO反应器中分离出三株好氧反硝化菌(分别命名为T3、T6、T7),经测定其革兰氏染色皆为阳性且均为杆状。通过分析形态学特征和16S rD-NA同源性比较,确定T3、T7为赤红红球菌属,T6为戈登氏菌属。菌株在以柠檬酸钠为碳源、硝酸钾为氮源的培养基中生长良好,将其按比例混合扩大培养后以5%的接种量投放于OGO反应器中,检测了菌株强化后反应器的脱氮效果。结果表明:聚乙烯醇(PVA)包埋菌泥投放较菌泥直接投放的处理效果好,经其强化后OGO反应器对COD、NH4+-N、TN的平均去除率分别为98.48%、90.18%、78.92%,比强化前分别提高了4.21%、6.43%、4.61%,且反应器出水中的NOx--N含量较低。Three strains of aerobic denitrifying bacteria were isolated from the OGO reactor with simultaneous nitrification and denitrification （SND） phenomenon, and named as T3, T6 and T7. They are all Gram-positive and rod-like. T3 and T7 are identified as Rhodococcus and T6 as Gordonia accord- ing to their morphological characteristics and 16S rDNA sequence homology comparison. The strains grow well in the culture medium with potassium nitrate as nitrogen source and sodium citrate as carbon source. After enlarging cultivation, the bacteria were added into the OGO reactor with inoculated amount of 5%. The nitrogen removal efficiency was tested in the OGO reactor enhanced with the aerobic denitrifying bac- teria. The results indicate that the bacteria immobilized by polyvinyl alcohol （PVA） are superior to free （non-immobilized） bacteria in treatment efficiency. The average removal rates of COD, ammonia nitro- gen and total nitrogen in the enhanced reactor are 98.48% , 90.18% and 78.92% respectively, being 4.21% , 6.43% and 4.61% higher than those in the non-enhanced reactor. Moreover, NO＋ -N in the effluent is lower.

关 键 词：OGO反应器 同步硝化反硝化 好氧反硝化 扩大培养 

分 类 号：X703[环境科学与工程—环境工程]