仿生壳聚糖基纳米纤维的自组装构建  

Fabrication of Biomimetic Chitosan-Based Nanofibers by Self-Assembly

作　　者：曾戎[1,2] 刘宏伟[1] 屠美[1,2] 赵剑豪[1,2] 查振刚[2,3] 周长忍[1,2] 

机构地区：[1]暨南大学材料科学与工程系 [2]暨南大学人工器官与材料教育部工程研究中心,广东广州510632 [3]暨南大学附属第一医院,广东广州510630

出　　处：《高分子材料科学与工程》2009年第12期122-125,共4页Polymer Materials Science & Engineering

基　　金：国家自然科学基金资助项目(20504018);国家高新技术发展计划(2007AA09Z440)

摘　　要：通过天然阳离子型高分子壳聚糖与三聚磷酸钠的静电自组装,制备模仿细胞外基质纳米结构的仿生壳聚糖基纳米纤维。研究了介质、壳聚糖浓度、反应物比例、添加胶原等因素对自组装复合产物的影响;并采用透射电镜、X射线衍射和红外光谱等技术表征了纳米纤维的结构。结果表明,介质对壳聚糖基纳米复合物的结构和形态的影响很大;以己二酸溶液为介质,在温和条件下,可获得结构可控的壳聚糖基纳米纤维,有望用于构建模仿天然细胞外基质的组织工程支架。Biomimetic polymeric nanofibers have received increasing interests for tissue engineering due to their structural similarity to extracellular matrix（ECM） recently.In this work,biomimetic chitosan-based nanofibers were successfully prepared by electrostatic self-assembly of chitosan and tripolyphosphate（TPP） with the help of adipic acid under mild conditions,and characterized using transmission electron microscopy（TEM）,X-ray diffraction（XRD） and Fourier-transform infrared（FT-IR） spectroscopy.Factors affecting the formation of nanofibers were investigated,including reaction medium,chitosan concentration,mixed ratio and the incorporation of collagen.It＇s found that the crystal structure,morphology and interaction of chitosan/TPP nanocomplexes are greatly affected by reaction medium.The results suggest that biomimetic chitosan/TPP nanofibers may be used in tissue engineering for providing a biomimetic microenvironment similar to the natural ECM for regenerative cells and tissues.

关 键 词：壳聚糖 静电自组装 仿生 纳米纤维 

分 类 号：TB383[一般工业技术—材料科学与工程]