用细胞筛选噬菌体抗体库分离自然杀伤细胞抑制受体的单链抗体  

Isolation of scFv fragments specific for natural killer cell inhibitory receptor using cells to select a phage display library

作　　者：乔旭刚[1] Unch.,ZH 

机构地区：[1]暨南大学医学院血液病研究室

出　　处：《中国病理生理杂志》1998年第5期500-503,共4页Chinese Journal of Pathophysiology

摘　　要：目的与方法：用Ｎｉｓｉｍ等噬菌体抗体库经细胞筛选，分离自然杀伤细胞抑制受体（ＮＫＡＴ１）的单链抗体。结果：８次筛选循环后，分离到的单链抗体不但可以特异性识别到表达在细胞表面的ＮＫＡＴ１分子，而且也能与纯化的ＮＫＡＴ１蛋白结合，这些单链抗体为３１ｋＤａ的分子。结论：用噬菌体抗体库可以简便。AIM and METHOD:“Nissim”phage display library was explored to isolate scFv fragments specific for natural killer cell inhibitory receptor (NKAT1) by cell panning.RESULTS:After 8 rounds of panning,the isolated scFv fragments were found to react not only with NKAT1 molecules expressed on the surface of cells,but also with purified NKAT1 protein.These scFv fragments were major bands of 31 kDa.CONCLUSION:Highly specific scFv fragments against natural killer cell inhibitory receptor can be obtained from a phage display library by relatively simple procedures.

关 键 词：杀伤细胞 抗体 流式细胞术 自然杀伤 SCFV 

分 类 号：R392－33[医药卫生—免疫学]