镁对大鼠缺氧再给氧心肌细胞内游离钙的影响  

The effect of magnesium on intracellular free calcium level in cultured neonatal rat anoxia-reoxygenation myocardial cells

作　　者：朱俏萍[1] 陈春艾 罗丽华 赖世忠[2] 刘伊丽[2] 

机构地区：[1]广州解放军157医院心血管内科 [2]第一军医大学南方医院心血管内科

出　　处：《中国病理生理杂志》1998年第5期508-508,共1页Chinese Journal of Pathophysiology

摘　　要：目的和方法：用ＡＣＡＳ５７０粘附式细胞仪，以荧光素染色法观察镁（Ｍｇ２＋）对体外培养乳鼠心肌细胞内游离钙（Ｃａ２＋）的影响及对缺氧再给氧时细胞内Ｃａ２＋作用。结果：细胞外Ｍｇ２＋降至０３ｍｍｏｌ·Ｌ－１，细胞内Ｃａ２＋荧光强度上升速度加快，达到稳定所需时间延长，出现Ｃａ２＋振荡曲线。增加Ｍｇ２＋浓度可使细胞内Ｃａ２＋降低。Ｍｇ２＋还可以显著减少缺氧再给氧时细胞内Ｃａ２＋，Ｐ＜００１。结论：细胞外低Ｍｇ２＋可导致细胞内Ｃａ２＋增加。AIM and METHODS:Adherent cell analysis and sorting cytometer 570 (ACAS 570) was used to study the effect of magnesium on intracellular free calcium (Ca 2+ ) level in cultured neonatal rat myocardial cells and in anoxia-reoxygenation myocardial cells with fluorescence staining method. RESULTS:Intracellular Ca 2+ raising speed was fast, stable time was delayed and appeared cytoplasmic calcium oscillation after megnesium decreased to 0 3mmol·L -1 . The level of intracellular Ca 2+ decreased immediately after the addition of magnesium. Magnesium reduced intracellular Ca 2+ level significantly due to anoxia-reoxygenation.CONCLUSIONS:The Magnesium in low concentration increased intracellular Ca 2+ , in normal myocardial cell magnesium could stabilize free Ca 2+ ,and against Ca 2+ overload during anoxi-reoxygenation.

关 键 词：钙 镁 心肌细胞 缺氧 大鼠 

分 类 号：R594.302[医药卫生—内科学] R542.202[医药卫生—临床医学]