2-甲氧雌二醇对HL60白血病细胞凋亡及Bcl-2蛋白表达的影响  

Effect of 2-Methoxyestradiol on Apoptosis of HL60 Cells and Expression of Bcl-2 Protein in vitro

作　　者：蔡应木[1] 张俏忻[1] 王雪华[1] 刘元生[2] 吴映娥[1] 焦晓阳[1] 

机构地区：[1]汕头大学医学院第一附属医院检验科,广东汕头515041 [2]汕头大学医学院第一附属医院血液科,广东汕头515041

出　　处：《汕头大学医学院学报》2009年第4期212-214,共3页Journal of Shantou University Medical College

基　　金：汕头市重点科技计划项目(汕府科[2007]76号)

摘　　要：目的:探讨2-甲氧雌二醇(2-ME)对HL60白血病细胞增殖及凋亡的影响。方法:用MTT法和流式细胞术检测2-ME对HL60的增殖抑制及促凋亡作用,用流式细胞技术检测Bcl-2蛋白的表达。结果:随着2-ME浓度升高及作用时间延长,HL60细胞增殖明显受抑制(P<0.05),细胞凋亡率明显增加(P<0.05),当2-ME浓度为50μmol/L时,细胞凋亡率达65.8%;2-ME(30μmol/L)作用72h后,Bcl-2的表达较对照组显著减少(P<0.05)。结论:2-ME对HL60细胞增殖有抑制及促凋亡作用,Bcl-2表达下调在此过程中可能发挥重要作用。Objective：To investigate the effect of 2-Methoxyestradiol（2-ME）on the proliferation and apoptosis of HL60 cells in vitro. Methods：After treatment with 2-ME,the cell proliferation was detected with MTT,and the apoptosis was determined with flow cytometry. Expression of Bcl-2 protein in HL60 cell was detected with flow cytometry. Results：The growth inhibitation rate exhibited significant increases（P〈0.05）with the increased dose and extended time. The cells apoptotic rate increased significantly after treatment with 2-ME respectively（P〈0.05）. The cells apoptotic rate was 65.8% after treatment with 2-ME（50 μmol/L）for 72 hours. Bcl-2 decreased significantly compared with control group after treatment with 2-ME（30 μmol/L）for 72 hours（P〈0.05）. Conclusion：2-ME can inhibit proliferation and induce apoptosis of HL60 in vitro. The down regulation of Bcl-2 may play an important role in the procedure.

关 键 词：2-甲氧雌二醇 HL60细胞 BCL-2蛋白 

分 类 号：R286.91[医药卫生—中药学] R965.1[医药卫生—中医学]