中国南海总合草苔虫中抗肿瘤活性成分研究——Ⅰ.Bryostatin 4～6的分离鉴定及其体外抗肿瘤活性  

STUDIES ON THE ANTINEOPLASTIC CONSTITUENTS FROM MARINE BRYOZOAN BUGULA NERITINA IN THE SOUTH CHINA SEA( I )

作　　者：林厚文[1] 姚新生[2] 易杨华 李翔 吴厚铭 釜野德明[4] 

机构地区：[1]第二军医大学药学院天然药物化学教研室,上海200433 [2]沈阳药科大学,沈阳110015 [3]上海有机化学研究所,上海200031 [4]日本神奈川大学理学部

出　　处：《中国海洋药物》1998年第3期1-6,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金(编号 29672050)

摘　　要：以中国南海海域大量生长的总合草苔虫为原料,将化学分离和活性追踪相结合,经溶媒提取和多种层析方法(Sephadex LH-20,ODS,HPLC等)分离,得到3个活性单体。采用高分辨核磁共振仪(600MHz)测定了它们的DQF-COSY、HMQC、TOCSY、NOESY、ROESY等二维相关谱。利用波谱解析和化学方法,分别将其鉴定为:bryostatin 4、bryostatin 5、bryostatin 6,并对其~1HNMR和^(13)CNMR数据做了订正。所有化合物均系首次从中国产总合草苔虫中获得,并在体外抗肿瘤活性筛选中都表现出显著的抗肿瘤活性。Three active compounds were isolated from the marine bryozoan Bugula neritina living in the South China Sea by bioassay-guiding isolation method with a combination of extraction with suitable solvent and multiple column chromatography(Sephadex LH-20, ODS and preparative HPLC). Their structures were assigned as known bryostatin 4, bryostatin 5 and bryostatin 6 by intensive analysis of the data of high resolution 2DNMR(600 MHz,DQF-COSY,TOCSY,HMQC and ROESY). All these compounds were obtained from this bryozoan in the South China Sea for the first time and showed significant antineoplastic activities in vitro.

关 键 词：总合草苔虫 抗肿瘤活性 草苔虫内酯 大环内脂 

分 类 号：R931.74[医药卫生—生药学] Q959.233[医药卫生—药学]