氯化高铁血红素对血红蛋白合成及红细胞生成素受体的诱导作用  

INDUCTION OF HEMOGLOBIN SYNTHESIS AND ERYTHROPOIETIN RECEPTOR EXPRESSION BY HEMIN

作　　者：王传清[1] 袁弥满[1] 

机构地区：[1]上海医科大学中山医院血液科

出　　处：《上海医科大学学报》1998年第5期357-359,共3页Journal of Fudan University(Medical Science)

摘　　要：目的研究氯化高铁血红素（Ｈｍ）对红系细胞（Ｋ５６２细胞）血红蛋白合成及红细胞生成素（ＥＰＯ）受体表达的诱导作用。方法采用不同剂量Ｈｍ对体外培养Ｋ５６２细胞进行诱导，用联苯胺染色对诱导细胞血红蛋白进行定性测定，通过放射性标记配基结合实验测定Ｈｍ诱导后Ｋ５６２细胞膜ＥＰＯ受体的变化。结果诱导组血红蛋白阳性细胞比例由对照组的３．５％增加到３４．３％（Ｐ＜０．０５）。Ｋ５６２细胞对ＥＰＯ的诱导作用不敏感，而经Ｈｍ体外诱导４８ｈ后，其对ＥＰＯ的敏感性明显增加。诱导后Ｋ５６２细胞膜ＥＰＯ受体的数量由对照组的３～８个／细胞，诱导组为６～２５个／细胞（Ｐ＜０．０１）。PURPOSE To study K562 cells' hemoglobin synthesis and its erythropoietin receptor expression induced by hemin. METHODS K562 cells were induced by different dose of hemin in vitro . Hemoglobin of K562 cells was measured by staining with benzidine. Erythropoietin receptors were measured by 125 I labeled rhEPO binding. RESULTS The percentage of Hb + K562 cells was only 3.5% in liquid culture without hemin induction. Treatment of K562 cells with hemin increased the percentage of Hb + cells (34.3% vs 3.5%, P<0.01 ). K562 cells expressed lower sensitivity ot EPO, and the sensitivity was increased by hemin induction. 125 I labeled rhEPO binding showed that K562 cells expressed a few number of high-affinity EPO receptors corres￣ponding to 3～8 receptors/cell ( K d=0.23～0.27nmol/L). Hemin treatment increased the receptor expression (16～25 receptors/cell vs 3～8 receptors/cell, P< 0.01). CONCLUSIONS Our results indicate that hemin is an erythroid differentiation inducer, and it increases sensitivity of K562 cells to EPO by increasing the amount of EPO receptors.

关 键 词：氯化高铁血红素 血红蛋白 EPO受体 贫血 

分 类 号：R556.05[医药卫生—血液循环系统疾病]