耳蜗毛细胞分离方法的改良  

A MODIFIED METHOD OF SEPARATING HAIR CELLS FROM MICE

作　　者：张勤修[1,2] 迟放鲁[1,2] 曹恒明 赵剑星 顾全保[1,2] 

机构地区：[1]上海医科大学眼耳鼻喉科医院耳鼻喉科 [2]中国科学院上海细胞生物研究所

出　　处：《上海医科大学学报》1998年第5期377-378,共2页Journal of Fudan University(Medical Science)

摘　　要：目的探讨耳蜗毛细胞的分离方法。方法选出生３周的ＳＤ大白鼠，雌雄兼用，在自制的外淋巴液中取出耳蜗基底膜，置于０．２５ｍｇ／ｍｌ木瓜蛋白酶中，３７℃３０ｍｉｎ消化后，洗去酶液，用钢针在普通解剖镜下剥离毛细胞并置倒置相差显微镜下观察，记录成活时间。结果每侧耳蜗可获２００左右成活的毛细胞，５ｈ后仍有７０％的毛细胞成活。结论用自制改良外淋巴液，并简化了操作过程，可以满意地获得形态。PURPOSE To develope a modified method of separating hair cells from mice. METHODS In the modified perilymph solution, the organ of corti was briefly incubated with papain and the hair cells were isolated from the tissue by mechanomanipulation under anotomy microscope. RESULTS Two hundred hair cells including IHC (inner hair cell) and OHC (outer hair cell) were obtained from each cochlea, and (with short term) approximately 70% of the cells retained good viability after 5 hrs' culture at room temperature. CONCLUSIONS Using the modified method the hair cells with good viability can be easily obtained.

关 键 词：耳蜗 毛细胞 分离 大白鼠 

分 类 号：R329.492.3[医药卫生—人体解剖和组织胚胎学]