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机构地区:[1]暨南大学医学病理教研室,广州510632 [2]中山医科大学组胚教研室,广州510089
出 处:《生物医学工程学杂志》1998年第3期214-217,共4页Journal of Biomedical Engineering
摘 要:促进内皮细胞在聚氨酯材料上粘附是实现人工心和人工血管内皮细胞化的关键一环。用荧光法观察了碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)对内皮细胞在聚氨酯材料上粘附的影响。结果表明,FN实验组和Ⅳ型胶原实验组的细胞粘附率分别71%和68-5%,而对照组分别是62-5%和55-25%,P均<0-05,表明bFGF能够促进内皮细胞在聚氨酯材料上的粘附。提示bFGF作为一种辅助因素加入培养基中将有助于实现人工心、血管聚氨酯材料的内皮细胞化。This study addressed the problem of how to promote the adherence of endothelial cells(EC) to polyurethane (PU).The influence of basic fibroblast growth factor (bFGF) on the adherence of EC to PU was observed by using the fluorescent method.The results showed that the attachment rates for two bFGF groups(PU precoated with fibronectin and PU precoated with collagen IV) were 71% and 68.5%,which were significantly higher than those (62.5% and 55.25%) for two control groups,respectively.These demonstrate that bFGF can promote EC attachment to PU, and suggest that bFGF can be used as a supplement factor in media for achievement of endothelialization on artificial heart and blood vessels.
分 类 号:R318.11[医药卫生—生物医学工程]
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