大豆凝集素与肉鸡小肠黏膜细胞结合规律的研究  

Binding Rule of Soybean Agglutinin to Small Intestinal Epithelia in Broiler

作　　者：王彬[1] 胡海霞[1] 孙青松[1] 王利民[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118

出　　处：《畜牧与饲料科学》2009年第9期15-17,共3页Animal Husbandry and Feed Science

基　　金：吉林农业大学博士启动资金(2007026)

摘　　要：该试验以75日龄爱拔益加肉鸡为试验对象,饲喂含有20%生大豆日粮。试验期后,取十二指肠、空肠前段、空肠中段、空肠后段、回肠制作石蜡切片,采用免疫组织化学SP法染色,利用图像分析仪分析大豆凝集素与肠上皮细胞结合情况。结果表明,在鸡的不同肠段,大豆凝集素与十二指肠和空肠前段结合的平均光密度值显著高于空肠中、空肠后和回肠(P<0.05);在相同肠段不同部位的细胞,肠绒毛的平均光密度值最高,且与隐窝和肠壁的差异显著(P<0.05)。这表明了肉鸡的不同肠段上皮细胞糖基化模式具有差异性。Arbor Acres breeders,at 75 days old,fed the diet containing 20 % raw soybean to investigate the binding rule of soybean agglutinin to the small intestinal epithelia. After two weeks,samples of small intestinal tissue were taken out containing duodenum,front-jejunum,middle-jejunum,behind-jejunumand ileum,and embedded in paraffin. Sections were stained by the SP immunohistochemistry to analyze the distribution of soybean agglutinin with the Image-ProPlus software. The results showed that the average optical values of soybean agglutinin binding with duodenum and front-jejunum were significantly higher than that of middle jejunum,behind-jejunum and ileum in the different intestine segments （P〈0.05）. Binding with intestinal vills was significantly higher than that of crypts and intestinal wall in the different sites of epthelia （P〈0.05）. It indicated that glycosylation pattern is different among epithelial of different intestine in broiler.

关 键 词：大豆凝集素 小肠 肉鸡 

分 类 号：S831.5[农业科学—畜牧学]